Ultrastructural analysis of ectopic synaptic boutons arising from peripherally regenerated primary afferent fibers

H. Richard Koerber, Karoly Mirnics, Anahid M. Kavookjian, Alan R. Light

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The central axons of peripherally regenerated Aβ primary sensory neurons were impaled in the dorsal columns of α-chloralose-anesthetized cats 9-12 mo after axotomy. The adequate peripheral stimulus was determined, and the afferent fibers intracellularly stimulated while simultaneously recording the resulting cord dorsum potentials (CDPs). Fibers that successfully had reinnervated the skin responded to light tactile stimulation, and evoked CDPs that suggested dorsally located boutons were stained intracellularly with horseradish peroxidase (HRP). Two HRP-stained regenerated Aβ afferent fibers were recovered that supported large numbers of axon collaterals and swellings in laminae 1, II(o), and II(i). Sections containing the ectopic collateral fibers and terminals in the superficial dorsal horn were embedded in plastic. Analyses of serial ultrathin sections revealed that ectopic projections from both regenerated fibers supported numerous synaptic boutons filled with clear round vesicles, a few large dense core vesicles (LDCVs) and several mitochondria (>3). All profiles examined in serial sections (19) formed one to three asymmetric axo-dendritic contacts. Unmyelinated portions of ectopic fibers giving rise to en passant and terminal boutons often contained numerous clear round vesicles. Several boutons (47%) received asymmetric contacts from axon terminals containing pleomorphic vesicles. These results strongly suggest that regenerated Aβ fibers activated by light tactile stimuli support functional connections in the superficial dorsal horn that have distinct ultrastructural features. In addition, the appearance of LDCVs suggests that primary sensory neurons are capable of changing their neurochemical phenotype.

Original languageEnglish (US)
Pages (from-to)1636-1644
Number of pages9
JournalJournal of Neurophysiology
Volume81
Issue number4
DOIs
StatePublished - Jan 1 1999

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Presynaptic Terminals
Secretory Vesicles
Touch
Sensory Receptor Cells
Horseradish Peroxidase
Axons
Axotomy
Myelinated Nerve Fibers
Chloralose
Plastics
Mitochondria
Cats
Phenotype
Skin
Spinal Cord Dorsal Horn

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

Ultrastructural analysis of ectopic synaptic boutons arising from peripherally regenerated primary afferent fibers. / Koerber, H. Richard; Mirnics, Karoly; Kavookjian, Anahid M.; Light, Alan R.

In: Journal of Neurophysiology, Vol. 81, No. 4, 01.01.1999, p. 1636-1644.

Research output: Contribution to journalArticle

Koerber, H. Richard ; Mirnics, Karoly ; Kavookjian, Anahid M. ; Light, Alan R. / Ultrastructural analysis of ectopic synaptic boutons arising from peripherally regenerated primary afferent fibers. In: Journal of Neurophysiology. 1999 ; Vol. 81, No. 4. pp. 1636-1644.
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