Ultrasound thrombolysis mediated by transcatheter injection of microbubble in vitro

Meng Jiao Guo, Shun Ji Gao, Juan Tu, Zheng Liu, Dong Zhang, Feng Xie

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the enhancement of ultrasound thrombolysis in vitro using transcatheter injection of microbubble. Methods: Fresh human blood clots (n=40) divided into experimental group 1 (n=10, transcatheter injection of microbubble and urokinase+ultrasound), experimental group 2 (n=10, peripheral circulation injection of urokinase and microbubbles+ultrasound), control group 1 (n=10, transcatheter injection of microbubble and urokinase), control group 2 (n=10, peripheral circulation injection of urokinase and microbubbles). Therapeutic US was operated at the frequency of 1.0 MHz with the peak negative pressure of 512 kPa. The thrombolysis rate of clot was calculated after treatment. US release of MBDiO was observed fluorescent distribution and intensity in thrombus after treatment. Results: The thrombolysis rate of experimental group 1 (137.70%±3.17%) was significantly higher than that of experimental group 2 (11.67%±1.13%), control group 1 (14.37%±2.22%) and control group 2 (7.90%±0.68%, all P<0.05). Transcatheter injection of microbubble usually developed clusters of strong echo in thrombus with acoustic shadow. After treatment, the echo was scattered and spread. Laser confocal microscopy found that a large amount of bright fluorescent particles in the clots in experimental group 1, but rare fluorescence could be found in the clots in experimental group 2. Conclusion: The thrombolysis rate of transcatheter injection of microbubble and urokinase+ultrasound can significantly higher than the method of peripheral circulation injection of urokinase and microbubbles.

Original languageEnglish (US)
Pages (from-to)169-173
Number of pages5
JournalChinese Journal of Interventional Imaging and Therapy
Volume12
Issue number3
DOIs
StatePublished - Mar 10 2015

Fingerprint

Microbubbles
Urokinase-Type Plasminogen Activator
Injections
Control Groups
Thrombosis
Confocal Microscopy
In Vitro Techniques
Therapeutics
Acoustics
Fluorescence
Pressure

Keywords

  • Thrombolysis
  • Thrombosis
  • Transcatheter injection of microbubbles

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Ultrasound thrombolysis mediated by transcatheter injection of microbubble in vitro. / Guo, Meng Jiao; Gao, Shun Ji; Tu, Juan; Liu, Zheng; Zhang, Dong; Xie, Feng.

In: Chinese Journal of Interventional Imaging and Therapy, Vol. 12, No. 3, 10.03.2015, p. 169-173.

Research output: Contribution to journalArticle

Guo, Meng Jiao ; Gao, Shun Ji ; Tu, Juan ; Liu, Zheng ; Zhang, Dong ; Xie, Feng. / Ultrasound thrombolysis mediated by transcatheter injection of microbubble in vitro. In: Chinese Journal of Interventional Imaging and Therapy. 2015 ; Vol. 12, No. 3. pp. 169-173.
@article{24f361404eb34fb794d69a6a9497cf34,
title = "Ultrasound thrombolysis mediated by transcatheter injection of microbubble in vitro",
abstract = "Objective: To investigate the enhancement of ultrasound thrombolysis in vitro using transcatheter injection of microbubble. Methods: Fresh human blood clots (n=40) divided into experimental group 1 (n=10, transcatheter injection of microbubble and urokinase+ultrasound), experimental group 2 (n=10, peripheral circulation injection of urokinase and microbubbles+ultrasound), control group 1 (n=10, transcatheter injection of microbubble and urokinase), control group 2 (n=10, peripheral circulation injection of urokinase and microbubbles). Therapeutic US was operated at the frequency of 1.0 MHz with the peak negative pressure of 512 kPa. The thrombolysis rate of clot was calculated after treatment. US release of MBDiO was observed fluorescent distribution and intensity in thrombus after treatment. Results: The thrombolysis rate of experimental group 1 (137.70{\%}±3.17{\%}) was significantly higher than that of experimental group 2 (11.67{\%}±1.13{\%}), control group 1 (14.37{\%}±2.22{\%}) and control group 2 (7.90{\%}±0.68{\%}, all P<0.05). Transcatheter injection of microbubble usually developed clusters of strong echo in thrombus with acoustic shadow. After treatment, the echo was scattered and spread. Laser confocal microscopy found that a large amount of bright fluorescent particles in the clots in experimental group 1, but rare fluorescence could be found in the clots in experimental group 2. Conclusion: The thrombolysis rate of transcatheter injection of microbubble and urokinase+ultrasound can significantly higher than the method of peripheral circulation injection of urokinase and microbubbles.",
keywords = "Thrombolysis, Thrombosis, Transcatheter injection of microbubbles",
author = "Guo, {Meng Jiao} and Gao, {Shun Ji} and Juan Tu and Zheng Liu and Dong Zhang and Feng Xie",
year = "2015",
month = "3",
day = "10",
doi = "10.13929/j.1672-8475.2015.03.010",
language = "English (US)",
volume = "12",
pages = "169--173",
journal = "Chinese Journal of Interventional Imaging and Therapy",
issn = "1672-8475",
publisher = "Editorial Office - Chinese Journal of Interventional",
number = "3",

}

TY - JOUR

T1 - Ultrasound thrombolysis mediated by transcatheter injection of microbubble in vitro

AU - Guo, Meng Jiao

AU - Gao, Shun Ji

AU - Tu, Juan

AU - Liu, Zheng

AU - Zhang, Dong

AU - Xie, Feng

PY - 2015/3/10

Y1 - 2015/3/10

N2 - Objective: To investigate the enhancement of ultrasound thrombolysis in vitro using transcatheter injection of microbubble. Methods: Fresh human blood clots (n=40) divided into experimental group 1 (n=10, transcatheter injection of microbubble and urokinase+ultrasound), experimental group 2 (n=10, peripheral circulation injection of urokinase and microbubbles+ultrasound), control group 1 (n=10, transcatheter injection of microbubble and urokinase), control group 2 (n=10, peripheral circulation injection of urokinase and microbubbles). Therapeutic US was operated at the frequency of 1.0 MHz with the peak negative pressure of 512 kPa. The thrombolysis rate of clot was calculated after treatment. US release of MBDiO was observed fluorescent distribution and intensity in thrombus after treatment. Results: The thrombolysis rate of experimental group 1 (137.70%±3.17%) was significantly higher than that of experimental group 2 (11.67%±1.13%), control group 1 (14.37%±2.22%) and control group 2 (7.90%±0.68%, all P<0.05). Transcatheter injection of microbubble usually developed clusters of strong echo in thrombus with acoustic shadow. After treatment, the echo was scattered and spread. Laser confocal microscopy found that a large amount of bright fluorescent particles in the clots in experimental group 1, but rare fluorescence could be found in the clots in experimental group 2. Conclusion: The thrombolysis rate of transcatheter injection of microbubble and urokinase+ultrasound can significantly higher than the method of peripheral circulation injection of urokinase and microbubbles.

AB - Objective: To investigate the enhancement of ultrasound thrombolysis in vitro using transcatheter injection of microbubble. Methods: Fresh human blood clots (n=40) divided into experimental group 1 (n=10, transcatheter injection of microbubble and urokinase+ultrasound), experimental group 2 (n=10, peripheral circulation injection of urokinase and microbubbles+ultrasound), control group 1 (n=10, transcatheter injection of microbubble and urokinase), control group 2 (n=10, peripheral circulation injection of urokinase and microbubbles). Therapeutic US was operated at the frequency of 1.0 MHz with the peak negative pressure of 512 kPa. The thrombolysis rate of clot was calculated after treatment. US release of MBDiO was observed fluorescent distribution and intensity in thrombus after treatment. Results: The thrombolysis rate of experimental group 1 (137.70%±3.17%) was significantly higher than that of experimental group 2 (11.67%±1.13%), control group 1 (14.37%±2.22%) and control group 2 (7.90%±0.68%, all P<0.05). Transcatheter injection of microbubble usually developed clusters of strong echo in thrombus with acoustic shadow. After treatment, the echo was scattered and spread. Laser confocal microscopy found that a large amount of bright fluorescent particles in the clots in experimental group 1, but rare fluorescence could be found in the clots in experimental group 2. Conclusion: The thrombolysis rate of transcatheter injection of microbubble and urokinase+ultrasound can significantly higher than the method of peripheral circulation injection of urokinase and microbubbles.

KW - Thrombolysis

KW - Thrombosis

KW - Transcatheter injection of microbubbles

UR - http://www.scopus.com/inward/record.url?scp=85014355084&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014355084&partnerID=8YFLogxK

U2 - 10.13929/j.1672-8475.2015.03.010

DO - 10.13929/j.1672-8475.2015.03.010

M3 - Article

AN - SCOPUS:85014355084

VL - 12

SP - 169

EP - 173

JO - Chinese Journal of Interventional Imaging and Therapy

JF - Chinese Journal of Interventional Imaging and Therapy

SN - 1672-8475

IS - 3

ER -