Ultrafine carbon black particles inhibit human lung fibroblast-mediated collagen gel contraction

Huijung Kim, Xiangde Liu, Tetsu Kobayashi, Tadashi Kohyama, Fu Qiang Wen, Debra J. Romberger, Heather Conner, Peter S. Gilmour, Kenneth Donaldson, William MacNee, Stephen I. Rennard

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Both acute and chronic exposure to particulates have been associated with increased mortality and morbidity from a number of causes, including chronic obstructive pulmonary disease and other chronic lung diseases. The current study evaluated the hypothesis that ultrafine carbon particles, a component of ambient particulates, could affect tissue repair. To assess this, the three-dimensional collagen gel contraction model was used. Ultrafine carbon black particles, but not fine carbon black, inhibited fibroblast-mediated collagen gel contraction. Although previous research has indicated that inflammatory effects of ultrafine carbon black particles are mediated by oxidant mechanisms, the current study suggests that ultrafine carbon black's inhibition of fibroblast gel contraction is mediated by the binding of both fibronectin and transforming growth factor (TGF)-β to the ultrafine particles. Binding of TGF-β was associated with a reduction in nuclear localization of Smads, indicative of inhibition of TGF-β signal transduction. There was also a decrease in fibronectin mRNA, consistent with a decrease in TGF-β-mediated response. Taken together, these results demonstrate the ability of ultrafine particles to contribute to altered tissue repair and extend the known mechanisms by which these biologically active particles exert their effects.

Original languageEnglish (US)
Pages (from-to)111-121
Number of pages11
JournalAmerican journal of respiratory cell and molecular biology
Volume28
Issue number1
DOIs
StatePublished - Jan 1 2003

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ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Kim, H., Liu, X., Kobayashi, T., Kohyama, T., Wen, F. Q., Romberger, D. J., Conner, H., Gilmour, P. S., Donaldson, K., MacNee, W., & Rennard, S. I. (2003). Ultrafine carbon black particles inhibit human lung fibroblast-mediated collagen gel contraction. American journal of respiratory cell and molecular biology, 28(1), 111-121. https://doi.org/10.1165/rcmb.4796