Ultra-rapid quantitative isolation of specific transfer ribonucleic acids a solid-phase method

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Abstract

The following procedure is used in a rapid isolation of a specific (isoacceptor) tRNA from a mixture of deacylated tRNAs: (1) Acylation with the amino acid corresponding to the desired specific tRNA (2) Reaction of the specifically acylated tRNA with a new bi-functional reagent (PAMBSYL) (3) Binding of the pambsylated tRNA to a sulfhydryl-containing resin (4) Release of the specific tRNA from the resin after removal of the non-reacted tRNA. Steps 3 and 4 can be carried out in 20 min. One ml of the resin can bind approximately 200 mg of reacted tRNA.

Original languageEnglish (US)
Pages (from-to)181-187
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume59
Issue number1
DOIs
StatePublished - Jul 10 1974

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Transfer RNA
Resins
Transfer RNA Aminoacylation
Acylation
Amino Acids

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ultra-rapid quantitative isolation of specific transfer ribonucleic acids a solid-phase method. / Goss, D. J.; Parkhurst, Lawrence J.

In: Biochemical and Biophysical Research Communications, Vol. 59, No. 1, 10.07.1974, p. 181-187.

Research output: Contribution to journalArticle

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