Ubiquitous Presence in Mammalian Cells of Enzymatic Activity Specifically Cleaving 8‐Hydroxyguanine‐containing DNA

Fuyumi Yamamoto, Hiroshi Kasai, Tadayoshi Bessho, Myung‐Hee ‐H Chung, Hideo Inoue, Eiko Ohtsuka, Tomokatsu Hori, Susumu Nishimura

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Abstract

Here we report the finding of enzymatic activity that specifically cleaves DNA containing 8‐hydroxyguanine (oh8Gua) residues in various mammalian cells. To detect this activity, we used a synthetic double‐stranded DNA containing a single oh8Gua at a defined position as the substrate, and analyzed the products of enzymatic digestion by polyacrylamide gel electrophoresis. Two cleavage sites near the oh8Gua residue were detected with partially purified fractions from cow brain and rat liver, and also with preparations from all mammalian tissues examined. These results suggest that enzymatic activity for the removal of oh8Gua from DNA is widely distributed in mammalian cells.

Original languageEnglish (US)
Pages (from-to)351-357
Number of pages7
JournalJapanese Journal of Cancer Research
Volume83
Issue number4
DOIs
StatePublished - Apr 1992

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Keywords

  • 8‐Hydroxyguanine
  • Key words
  • Repair enzyme

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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