Two enzymes, BtaA and BtaB, are sufficient for betaine lipid biosynthesis in bacteria

Wayne Riekhof, Carl Andre, Christoph Benning

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Betaine lipids are non-phosphorous glycerolipid analogs of phosphatidylcholine. The biosynthesis of the betaine lipid diacylglyceryl-N,N,N- trimethylhomoserine has previously been studied in phosphate-starved cells of the purple bacterium Rhodobacter sphaeroides, and a genetic approach identified two proteins that are necessary for this process. Here, we show that all reactions of DGTS biosynthesis in R. sphaeroides are attributable to RsBtaA and RsBtaB, as co-expression of the respective genes leads to DGTS formation in Escherichia coli, which normally lacks this lipid. The recombinant RsBtaA protein was membrane-associated and showed S-adenosylmethionine/diacylglycerol 3-amino-3-carboxypropyl transferase activity. RsBtaA directed the transfer of label from 1-[14C]S-adenosylmethionine or [14C] diacylglycerol at equal rates into the betaine lipid precursor diacylglycerylhomoserine identifying both metabolites as the substrates of the reaction. Comparative analysis of RsBtaA and its bacterial orthologs revealed a motif with similarity to the AdoMet binding pocket of methyltransferases, and allowed the prediction of residues involved in substrate binding.

Original languageEnglish (US)
Pages (from-to)96-105
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume441
Issue number1
DOIs
StatePublished - Sep 1 2005

Fingerprint

Betaine
Biosynthesis
S-Adenosylmethionine
Bacteria
Lipids
Rhodobacter sphaeroides
Diglycerides
Enzymes
Proteobacteria
Methyltransferases
Substrates
Transferases
Metabolites
Phosphatidylcholines
Recombinant Proteins
Escherichia coli
Labels
Genes
Phosphates
Membranes

Keywords

  • Betaine lipid
  • DAG metabolism
  • Membrane associated enzyme
  • Membrane biogenesis
  • Methyltransferase
  • Non-phosphorous lipid
  • One carbon metabolism
  • Phosphate starvation
  • Rhodobacter sphaeroides
  • SAM metabolism

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Two enzymes, BtaA and BtaB, are sufficient for betaine lipid biosynthesis in bacteria. / Riekhof, Wayne; Andre, Carl; Benning, Christoph.

In: Archives of Biochemistry and Biophysics, Vol. 441, No. 1, 01.09.2005, p. 96-105.

Research output: Contribution to journalArticle

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