Two-dimensional gels

An easy method for large quantities of proteins

Richard L. Barent, Thomas E Elthon

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A new two-dimensional gel technique has been developed that makes it possible to load large amounts of protein and still retain good resolution. Protein samples are added to the isoelectric-focusing acrylamide solution prior to polymerization. Focusing times are short, making it possible to complete both dimensions of a two-dimensional gel in five to six hours. The technique yields larger amounts of separated protein for sequencing, and also greatly increases the quality of two-dimensional immunoblots.

Original languageEnglish (US)
Pages (from-to)338-344
Number of pages7
JournalPlant Molecular Biology Reporter
Volume10
Issue number4
DOIs
StatePublished - Nov 1 1992

Fingerprint

Gels
gels
Acrylamide
Protein Sequence Analysis
Isoelectric Focusing
Polymerization
Proteins
proteins
acrylamides
isoelectric focusing
polymerization
methodology
sampling

Keywords

  • Two-dimensional gels
  • protein sequencing

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

Cite this

Two-dimensional gels : An easy method for large quantities of proteins. / Barent, Richard L.; Elthon, Thomas E.

In: Plant Molecular Biology Reporter, Vol. 10, No. 4, 01.11.1992, p. 338-344.

Research output: Contribution to journalArticle

Barent, Richard L. ; Elthon, Thomas E. / Two-dimensional gels : An easy method for large quantities of proteins. In: Plant Molecular Biology Reporter. 1992 ; Vol. 10, No. 4. pp. 338-344.
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