Turnip crinkle virus defective interfering RNAs intensify viral symptoms and are generated de novo

X. H. Li, L. A. Heaton, T. J. Morris, A. E. Simon

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Defective interfering (DI) RNAs have been isolated from a broad spectrum of animal viruses and have recently been identified in plant virus infections. Because of their ubiquitous nature, DIs are thought to play an important role in virus replication and yields. DI RNAs have now been found in association with a natural isolate of turnip crinkle virus (TCV-B) and are generated de novo after inoculation of turnip with virus derived from cloned transcripts. DI RNA G, naturally found in the TCV-B isolate, is a mosaic molecule with 5' and 3' viral segments and a repeat of 36 nucleotides at the beginning of the 3' segment. The 5'-terminal 21 nucleotides of DI RNA G were not similar to genomic TCV sequences but did resemble sequences found at the 5' end of other small RNAs associated with TCV (satellite RNAs). DI RNA G interferes with the accumulation of TCV genomic RNA and, unlike other DI RNAs, intensifies the symptoms of its helper virus. Infection of turnip with virus derived from cloned transcripts of TCV-B resulted in de novo generation of a DI RNA, DI1 RNA. DI1 RNA differed from DI RNA G by containing exact 5' and 3' ends of TCV as well as an internal virus segment.

Original languageEnglish (US)
Pages (from-to)9173-9177
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number23
DOIs
StatePublished - Dec 22 1989

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Carmovirus
Viral RNA
RNA
Viruses
Brassica napus
Satellite RNAs
Nucleotides
Helper Viruses
Plant Viruses
Virus Diseases
Virus Replication

Keywords

  • plant virus
  • satellite RNAs

ASJC Scopus subject areas

  • General

Cite this

Turnip crinkle virus defective interfering RNAs intensify viral symptoms and are generated de novo. / Li, X. H.; Heaton, L. A.; Morris, T. J.; Simon, A. E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 23, 22.12.1989, p. 9173-9177.

Research output: Contribution to journalArticle

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