Tuning the mechanical properties of bioreducible multilayer films for improved cell adhesion and transfection activity

Jenifer Blacklock, Andreas Vetter, Andreas Lankenau, David Oupický, Helmuth Möhwald

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A simple approach to the mechanical modulation of layer-by-layer (LbL) films is through manipulation of the film assembly. Here, we report results based on altering the salt concentration during film assembly and its effect on film rigidity. Based on changes in film rigidity, cell adhesion characteristics and transfection activity were investigated in vitro. LbL films consisting of reducible hyperbranched poly(amide amine) (RHB) have been implemented along with DNA for investigating fibroblast adhesion on [RHB/DNA]n/2 films with varying rigidities. The rigidity was varied by changing the ionic concentration of the deposition solution between 0.01 m NaCl and 1.0 m NaCl. Molecular force probe (MFP) measurements were performed to measure the apparent Young's modulus, EAPP, of the films in situ. Cell adhesion and stress-fiber characteristics were investigated using total internal reflectance microscopy (TIRF-M). The average cell peripheral area, fiber density and average fiber length during 5 days of cell growth on films with either low (below 2.0 MPa) or high (above 2.0 MPa) film elastic modulus were investigated. Transfection studies were performed using gfpDNA and SEAP-DNA to investigate if changes in cell adhesion affect transfection activity. Furthermore, cell proliferation and cytotoxicity studies were used to investigate cellular viability over a week. The results have shown that surface modification of bioreducible LbL films of controlled thickness and roughness promotes cellular adhesion, stress-fiber growth and increased transfection activity without the need for an additional adhesive protein pre-coating of the surface or chemical cross-linking of the film.

Original languageEnglish (US)
Pages (from-to)7167-7174
Number of pages8
JournalBiomaterials
Volume31
Issue number27
DOIs
StatePublished - Sep 1 2010

Fingerprint

Multilayer films
Cell adhesion
Cell Adhesion
Transfection
Tuning
Mechanical properties
Stress Fibers
Elastic Modulus
DNA
Molecular Probes
Rigidity
Growth
Amides
Adhesives
Amines
Microscopy
Fibers
Fibroblasts
Salts
Cell Proliferation

Keywords

  • Cell adhesion
  • Layer-by-layer, LbL
  • Molecular force probe, MFP
  • Stress-fiber orientation
  • TIRF-M
  • Transfection

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Tuning the mechanical properties of bioreducible multilayer films for improved cell adhesion and transfection activity. / Blacklock, Jenifer; Vetter, Andreas; Lankenau, Andreas; Oupický, David; Möhwald, Helmuth.

In: Biomaterials, Vol. 31, No. 27, 01.09.2010, p. 7167-7174.

Research output: Contribution to journalArticle

Blacklock, Jenifer ; Vetter, Andreas ; Lankenau, Andreas ; Oupický, David ; Möhwald, Helmuth. / Tuning the mechanical properties of bioreducible multilayer films for improved cell adhesion and transfection activity. In: Biomaterials. 2010 ; Vol. 31, No. 27. pp. 7167-7174.
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