Tumor uptake and mitotic activity pattern of 5-[125I]iodo-2′-deoxyuridine after intravesical infusion in patients with bladder cancer

Giuliano Mariani, Paola Collecchi, Sergio Baldassarri, Lorella Di Luca, Simona Buralli, Gabriella Fontanini, Janina Baranowska-Kortylewicz, S. James Adelstein, Amin I. Kassis

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Abstract

In patients with bladder cancer, little is known about diffusion in the tumor mass of 5-iodo-2′-deoxyuridine (IUdR) administered intraluminally, although previous studies based on external scanning have shown promising tumor-targeting properties of IUdR instilled intravesically. This study compared the pattern of IUdR uptake by bladder cancer cells with the actual distribution of mitotic activity, as evaluated by incubation of ex vivo tumor specimens with tritiated thymidine. Methods: The [125I]IUdR (2-13 MBq) was instilled over 1-3 hr in the bladder of four patients with bladder cancer scheduled for ablative surgery. Twenty-four hours later, surgical samples were assayed for radioactivity and processed for microautoradiography, while fresh tumor specimens were fragmented, incubated with [3H]thymidine and further processed for microautoradiography. The diffusion of labeled IUdR across the bladder wall was evaluated by blood sampling. Results: Tumor incorporation of [125I]IUdR 24 hr after intravesical instillation was 0.002%-0.05% ID/g, while the average tumor-to-normal bladder ratio was about 20. Microautoradiography showed that [125I]IUdR incorporation was confined to tumor cells in the most superficial layers of the bladder, while incubation of the tumor fragments with [3H]thymidine demonstrated the presence of diffuse mitotic activity also in the deeper tumor mass. Diffusion of labeled IUdR in the general circulation was minimal. Conclusion: Poor diffusion in the tumor mass makes *IUdR unsuitable for intracavitary therapy of bladder cancer, but the role of such an approach in the postsurgical "sterilization" of cancer remnants floating in the bladder lumen after partial cystectomy should be explored.

Original languageEnglish (US)
Pages (from-to)16S-19S
JournalJournal of Nuclear Medicine
Volume37
Issue number4 SUPPL.
StatePublished - Apr 1 1996

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Deoxyuridine
Idoxuridine
Urinary Bladder Neoplasms
Neoplasms
Urinary Bladder
Thymidine
Intravesical Administration
Cystectomy
Radioactivity

Keywords

  • Bladder cancer
  • Intravesical instillation
  • Iodine-125-IUdR
  • Locoregional administration

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Mariani, G., Collecchi, P., Baldassarri, S., Di Luca, L., Buralli, S., Fontanini, G., ... Kassis, A. I. (1996). Tumor uptake and mitotic activity pattern of 5-[125I]iodo-2′-deoxyuridine after intravesical infusion in patients with bladder cancer. Journal of Nuclear Medicine, 37(4 SUPPL.), 16S-19S.

Tumor uptake and mitotic activity pattern of 5-[125I]iodo-2′-deoxyuridine after intravesical infusion in patients with bladder cancer. / Mariani, Giuliano; Collecchi, Paola; Baldassarri, Sergio; Di Luca, Lorella; Buralli, Simona; Fontanini, Gabriella; Baranowska-Kortylewicz, Janina; Adelstein, S. James; Kassis, Amin I.

In: Journal of Nuclear Medicine, Vol. 37, No. 4 SUPPL., 01.04.1996, p. 16S-19S.

Research output: Contribution to journalArticle

Mariani, G, Collecchi, P, Baldassarri, S, Di Luca, L, Buralli, S, Fontanini, G, Baranowska-Kortylewicz, J, Adelstein, SJ & Kassis, AI 1996, 'Tumor uptake and mitotic activity pattern of 5-[125I]iodo-2′-deoxyuridine after intravesical infusion in patients with bladder cancer', Journal of Nuclear Medicine, vol. 37, no. 4 SUPPL., pp. 16S-19S.
Mariani G, Collecchi P, Baldassarri S, Di Luca L, Buralli S, Fontanini G et al. Tumor uptake and mitotic activity pattern of 5-[125I]iodo-2′-deoxyuridine after intravesical infusion in patients with bladder cancer. Journal of Nuclear Medicine. 1996 Apr 1;37(4 SUPPL.):16S-19S.
Mariani, Giuliano ; Collecchi, Paola ; Baldassarri, Sergio ; Di Luca, Lorella ; Buralli, Simona ; Fontanini, Gabriella ; Baranowska-Kortylewicz, Janina ; Adelstein, S. James ; Kassis, Amin I. / Tumor uptake and mitotic activity pattern of 5-[125I]iodo-2′-deoxyuridine after intravesical infusion in patients with bladder cancer. In: Journal of Nuclear Medicine. 1996 ; Vol. 37, No. 4 SUPPL. pp. 16S-19S.
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abstract = "In patients with bladder cancer, little is known about diffusion in the tumor mass of 5-iodo-2′-deoxyuridine (IUdR) administered intraluminally, although previous studies based on external scanning have shown promising tumor-targeting properties of IUdR instilled intravesically. This study compared the pattern of IUdR uptake by bladder cancer cells with the actual distribution of mitotic activity, as evaluated by incubation of ex vivo tumor specimens with tritiated thymidine. Methods: The [125I]IUdR (2-13 MBq) was instilled over 1-3 hr in the bladder of four patients with bladder cancer scheduled for ablative surgery. Twenty-four hours later, surgical samples were assayed for radioactivity and processed for microautoradiography, while fresh tumor specimens were fragmented, incubated with [3H]thymidine and further processed for microautoradiography. The diffusion of labeled IUdR across the bladder wall was evaluated by blood sampling. Results: Tumor incorporation of [125I]IUdR 24 hr after intravesical instillation was 0.002{\%}-0.05{\%} ID/g, while the average tumor-to-normal bladder ratio was about 20. Microautoradiography showed that [125I]IUdR incorporation was confined to tumor cells in the most superficial layers of the bladder, while incubation of the tumor fragments with [3H]thymidine demonstrated the presence of diffuse mitotic activity also in the deeper tumor mass. Diffusion of labeled IUdR in the general circulation was minimal. Conclusion: Poor diffusion in the tumor mass makes *IUdR unsuitable for intracavitary therapy of bladder cancer, but the role of such an approach in the postsurgical {"}sterilization{"} of cancer remnants floating in the bladder lumen after partial cystectomy should be explored.",
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AU - Buralli, Simona

AU - Fontanini, Gabriella

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