Tryptophan 375 stabilizes the outer-domain core of gp120 for HIV vaccine immunogen design

Duoyi Hu, Dane Bowder, Wenzhong Wei, Jesse Thompson, Mark A Wilson, Shi-Hua Xiang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The outer-domain core of gp120 may serve as a better HIV vaccine immunogen than the full-length gp120 because of its greater stability and immunogenicity. In our previous report, we introduced two disulfide bonds to the outer-domain core of gp120 to fix its conformation into a CD4-bound state, which resulted in a significant increase in its immunogenicity when compared to the wild-type outer-domain core. In this report, to further improve the immunogenicity of the outer-domain core based immunogen, we have introduced a Tryptophan residue at gp120 amino acid sequence position 375 (375S/W). Our data from immunized guinea pigs indeed shows a striking increase in the immune response due to this stabilized core outer-domain. Therefore, we conclude that the addition of 375W to the outer-domain core of gp120 further stabilizes the structure of immunogen and increases the immunogenicity.

Original languageEnglish (US)
Pages (from-to)3067-3075
Number of pages9
JournalVaccine
Volume35
Issue number23
DOIs
StatePublished - May 25 2017

Fingerprint

HIV Envelope Protein gp120
AIDS Vaccines
Tryptophan
Disulfides
tryptophan
Amino Acid Sequence
Guinea Pigs
immune response
vaccines
antigens
disulfide bonds
guinea pigs
amino acid sequences

Keywords

  • 375W
  • Gp120
  • HIV-1 subtype C
  • Immunogenicity
  • Outer domain (OD)
  • Structure-based design
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Tryptophan 375 stabilizes the outer-domain core of gp120 for HIV vaccine immunogen design. / Hu, Duoyi; Bowder, Dane; Wei, Wenzhong; Thompson, Jesse; Wilson, Mark A; Xiang, Shi-Hua.

In: Vaccine, Vol. 35, No. 23, 25.05.2017, p. 3067-3075.

Research output: Contribution to journalArticle

Hu, Duoyi ; Bowder, Dane ; Wei, Wenzhong ; Thompson, Jesse ; Wilson, Mark A ; Xiang, Shi-Hua. / Tryptophan 375 stabilizes the outer-domain core of gp120 for HIV vaccine immunogen design. In: Vaccine. 2017 ; Vol. 35, No. 23. pp. 3067-3075.
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