Triticum mosaic poacevirus enlists P1 rather than HC-Pro to suppress RNA silencing-mediated host defense

Satyanarayana Tatineni, Feng Qu, Ruhui Li, T. Jack Morris, Roy French

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Triticum mosaic virus (TriMV) is the type species of the newly established Poacevirus genus in the family Potyviridae. In this study, we demonstrate that in contrast to the helper component-proteinase (HC-Pro) of Potyvirus species, the P1 proteins of TriMV and Sugarcane streak mosaic poacevirus function in suppression of RNA silencing (SRS). TriMV P1 effectively suppressed silencing induced by single- or double-stranded RNAs (ss/ds RNAs), and disrupted the systemic spread of silencing signals at a step after silencing signal production. Interestingly, contrary to enhanced SRS activity of potyviral HC-Pro by co-expression with P1, the presence of TriMV HC-Pro reduced SRS activity of TriMV P1. Furthermore, TriMV P1 suppressed systemic silencing triggered by dsRNA more efficiently than the HC-Pro of Turnip mosaic potyvirus. Furthermore, TriMV P1 enhanced the pathogenicity of a heterologous virus. Our results established poaceviral P1 as a potent RNA silencing suppressor that probably employs a novel mechanism to suppress RNA silencing-based antiviral defense.

Original languageEnglish (US)
Pages (from-to)104-115
Number of pages12
JournalVirology
Volume433
Issue number1
DOIs
StatePublished - Nov 10 2012

Fingerprint

Mosaic Viruses
RNA Interference
Triticum
Peptide Hydrolases
Potyviridae
Potyvirus
Brassica napus
Viral Structures
Saccharum
Double-Stranded RNA
Antiviral Agents
Virulence
Viruses

Keywords

  • HC-Pro
  • Inhibition
  • P1
  • Poacevirus
  • Potyviridae
  • RNA silencing suppressor
  • Sugarcane streak mosaic virus
  • Systemic silencing
  • Wheat

ASJC Scopus subject areas

  • Virology

Cite this

Triticum mosaic poacevirus enlists P1 rather than HC-Pro to suppress RNA silencing-mediated host defense. / Tatineni, Satyanarayana; Qu, Feng; Li, Ruhui; Jack Morris, T.; French, Roy.

In: Virology, Vol. 433, No. 1, 10.11.2012, p. 104-115.

Research output: Contribution to journalArticle

Tatineni, Satyanarayana ; Qu, Feng ; Li, Ruhui ; Jack Morris, T. ; French, Roy. / Triticum mosaic poacevirus enlists P1 rather than HC-Pro to suppress RNA silencing-mediated host defense. In: Virology. 2012 ; Vol. 433, No. 1. pp. 104-115.
@article{e4e7ea15ccf0458f906772623b5a9c1b,
title = "Triticum mosaic poacevirus enlists P1 rather than HC-Pro to suppress RNA silencing-mediated host defense",
abstract = "Triticum mosaic virus (TriMV) is the type species of the newly established Poacevirus genus in the family Potyviridae. In this study, we demonstrate that in contrast to the helper component-proteinase (HC-Pro) of Potyvirus species, the P1 proteins of TriMV and Sugarcane streak mosaic poacevirus function in suppression of RNA silencing (SRS). TriMV P1 effectively suppressed silencing induced by single- or double-stranded RNAs (ss/ds RNAs), and disrupted the systemic spread of silencing signals at a step after silencing signal production. Interestingly, contrary to enhanced SRS activity of potyviral HC-Pro by co-expression with P1, the presence of TriMV HC-Pro reduced SRS activity of TriMV P1. Furthermore, TriMV P1 suppressed systemic silencing triggered by dsRNA more efficiently than the HC-Pro of Turnip mosaic potyvirus. Furthermore, TriMV P1 enhanced the pathogenicity of a heterologous virus. Our results established poaceviral P1 as a potent RNA silencing suppressor that probably employs a novel mechanism to suppress RNA silencing-based antiviral defense.",
keywords = "HC-Pro, Inhibition, P1, Poacevirus, Potyviridae, RNA silencing suppressor, Sugarcane streak mosaic virus, Systemic silencing, Wheat",
author = "Satyanarayana Tatineni and Feng Qu and Ruhui Li and {Jack Morris}, T. and Roy French",
year = "2012",
month = "11",
day = "10",
doi = "10.1016/j.virol.2012.07.016",
language = "English (US)",
volume = "433",
pages = "104--115",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Triticum mosaic poacevirus enlists P1 rather than HC-Pro to suppress RNA silencing-mediated host defense

AU - Tatineni, Satyanarayana

AU - Qu, Feng

AU - Li, Ruhui

AU - Jack Morris, T.

AU - French, Roy

PY - 2012/11/10

Y1 - 2012/11/10

N2 - Triticum mosaic virus (TriMV) is the type species of the newly established Poacevirus genus in the family Potyviridae. In this study, we demonstrate that in contrast to the helper component-proteinase (HC-Pro) of Potyvirus species, the P1 proteins of TriMV and Sugarcane streak mosaic poacevirus function in suppression of RNA silencing (SRS). TriMV P1 effectively suppressed silencing induced by single- or double-stranded RNAs (ss/ds RNAs), and disrupted the systemic spread of silencing signals at a step after silencing signal production. Interestingly, contrary to enhanced SRS activity of potyviral HC-Pro by co-expression with P1, the presence of TriMV HC-Pro reduced SRS activity of TriMV P1. Furthermore, TriMV P1 suppressed systemic silencing triggered by dsRNA more efficiently than the HC-Pro of Turnip mosaic potyvirus. Furthermore, TriMV P1 enhanced the pathogenicity of a heterologous virus. Our results established poaceviral P1 as a potent RNA silencing suppressor that probably employs a novel mechanism to suppress RNA silencing-based antiviral defense.

AB - Triticum mosaic virus (TriMV) is the type species of the newly established Poacevirus genus in the family Potyviridae. In this study, we demonstrate that in contrast to the helper component-proteinase (HC-Pro) of Potyvirus species, the P1 proteins of TriMV and Sugarcane streak mosaic poacevirus function in suppression of RNA silencing (SRS). TriMV P1 effectively suppressed silencing induced by single- or double-stranded RNAs (ss/ds RNAs), and disrupted the systemic spread of silencing signals at a step after silencing signal production. Interestingly, contrary to enhanced SRS activity of potyviral HC-Pro by co-expression with P1, the presence of TriMV HC-Pro reduced SRS activity of TriMV P1. Furthermore, TriMV P1 suppressed systemic silencing triggered by dsRNA more efficiently than the HC-Pro of Turnip mosaic potyvirus. Furthermore, TriMV P1 enhanced the pathogenicity of a heterologous virus. Our results established poaceviral P1 as a potent RNA silencing suppressor that probably employs a novel mechanism to suppress RNA silencing-based antiviral defense.

KW - HC-Pro

KW - Inhibition

KW - P1

KW - Poacevirus

KW - Potyviridae

KW - RNA silencing suppressor

KW - Sugarcane streak mosaic virus

KW - Systemic silencing

KW - Wheat

UR - http://www.scopus.com/inward/record.url?scp=84866165401&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866165401&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2012.07.016

DO - 10.1016/j.virol.2012.07.016

M3 - Article

C2 - 22877841

AN - SCOPUS:84866165401

VL - 433

SP - 104

EP - 115

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -