Tricarboxylic acid cycle-dependent regulation of Staphylococcus epidermidis polysaccharide intercellular adhesin synthesis

Marat R. Sadykov, Michael E. Olson, Steven Halouska, Yefei Zhu, Paul D Fey, Robert Powers, Greg A Somerville

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Staphylococcus epidermidis is a major nosocomial pathogen primarily infecting immunocompromised individuals or those with implanted biomaterials (e.g., catheters). Biomaterial-associated infections often involve the formation of a biofilm on the surface of the medical device. In S. epidermidis, polysaccharide intercellular adhesin (PIA) is an important mediator of biofilm formation and pathogenesis. Synthesis of PIA is regulated by at least three DNA binding proteins (IcaR, SarA, and σB) and several environmental and nutritional conditions. Previously, we observed the environmental conditions that increased PIA synthesis decreased tricarboxylic acid (TCA) cycle activity. In this study, S. epidermidis TCA cycle mutants were constructed, and the function of central metabolism in PIA biosynthesis was examined. TCA cycle inactivation altered the metabolic status of S. epidermidis, resulting in a massive derepression of PIA biosynthetic genes and a redirection of carbon from growth into PIA biosynthesis. These data demonstrate that the bacterial metabolic status is a critical regulatory determinant of PIA synthesis. In addition, these data lead us to propose that the TCA cycle acts as a signal transduction pathway to translate external environmental cues into intracellular metabolic signals that modulate the activity of transcriptional regulators.

Original languageEnglish (US)
Pages (from-to)7621-7632
Number of pages12
JournalJournal of bacteriology
Volume190
Issue number23
DOIs
StatePublished - Dec 1 2008

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Citric Acid Cycle
Staphylococcus epidermidis
Biocompatible Materials
Biofilms
Iron-Dextran Complex
DNA-Binding Proteins
polysaccharide intercellular adhesin
Cues
Signal Transduction
Carbon
Catheters
Equipment and Supplies
Growth
Infection
Genes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Tricarboxylic acid cycle-dependent regulation of Staphylococcus epidermidis polysaccharide intercellular adhesin synthesis. / Sadykov, Marat R.; Olson, Michael E.; Halouska, Steven; Zhu, Yefei; Fey, Paul D; Powers, Robert; Somerville, Greg A.

In: Journal of bacteriology, Vol. 190, No. 23, 01.12.2008, p. 7621-7632.

Research output: Contribution to journalArticle

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