Triacylglyceride metabolism by Fusarium graminearum during colonization and sexual development on wheat

John C. Guenther, Heather E. Hallen-Adams, Heike Bücking, Yair Shachar-Hill, Frances Trail

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Fusarium graminearum, a devastating pathogen of small grains, overwinters on crop residues and produces ephemeral perithecia. Accumulation of lipids in overwintering hyphae would provide reserves for overwinter survival and perithecium development. Fatty acid composition of cultures during perithecium development indicated a drop in neutral lipid levels during development but little change in fatty acid composition across stages. Microscopic examination of cultures early in sexual development revealed hyphal cells engorged with lipid bodies. In comparison, vegetative hyphae contained few lipid bodies. Microarray analysis was performed on wheat stems at stages of colonization through perithecium development. Gene expression analysis during stages of perithecium development both in planta and in vitro (previously published) supports the view that lipid biosynthesis occurs during early stages of wheat colonization leading to sexual development and that lipid oxidation occurs as perithecia are developing. Analysis of gene expression during the stages of wheat stem colonization also revealed sets of genes unique to these stages. These results support the view that lipids accumulate in hyphae colonizing wheat stalks and are subsequently used in perithecium formation on stalk tissue. These results indicate that extensive colonization of plant tissue prior to harvest is essential for subsequent sporulation on crop residues and, thus, has important implications for inoculum reduction.

Original languageEnglish (US)
Pages (from-to)1492-1503
Number of pages12
JournalMolecular Plant-Microbe Interactions
Volume22
Issue number12
DOIs
StatePublished - Dec 1 2009

Fingerprint

Sexual Development
sexual development
Fusarium graminearum
Fusarium
Triticum
Hyphae
Lipids
wheat
metabolism
hyphae
lipid bodies
Fatty Acids
crop residues
Gene Expression
lipids
fatty acid composition
Microarray Analysis
gene expression
stems
Plantae

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

Triacylglyceride metabolism by Fusarium graminearum during colonization and sexual development on wheat. / Guenther, John C.; Hallen-Adams, Heather E.; Bücking, Heike; Shachar-Hill, Yair; Trail, Frances.

In: Molecular Plant-Microbe Interactions, Vol. 22, No. 12, 01.12.2009, p. 1492-1503.

Research output: Contribution to journalArticle

Guenther, John C. ; Hallen-Adams, Heather E. ; Bücking, Heike ; Shachar-Hill, Yair ; Trail, Frances. / Triacylglyceride metabolism by Fusarium graminearum during colonization and sexual development on wheat. In: Molecular Plant-Microbe Interactions. 2009 ; Vol. 22, No. 12. pp. 1492-1503.
@article{426a2516332743b78cba1d89a40a3bf1,
title = "Triacylglyceride metabolism by Fusarium graminearum during colonization and sexual development on wheat",
abstract = "Fusarium graminearum, a devastating pathogen of small grains, overwinters on crop residues and produces ephemeral perithecia. Accumulation of lipids in overwintering hyphae would provide reserves for overwinter survival and perithecium development. Fatty acid composition of cultures during perithecium development indicated a drop in neutral lipid levels during development but little change in fatty acid composition across stages. Microscopic examination of cultures early in sexual development revealed hyphal cells engorged with lipid bodies. In comparison, vegetative hyphae contained few lipid bodies. Microarray analysis was performed on wheat stems at stages of colonization through perithecium development. Gene expression analysis during stages of perithecium development both in planta and in vitro (previously published) supports the view that lipid biosynthesis occurs during early stages of wheat colonization leading to sexual development and that lipid oxidation occurs as perithecia are developing. Analysis of gene expression during the stages of wheat stem colonization also revealed sets of genes unique to these stages. These results support the view that lipids accumulate in hyphae colonizing wheat stalks and are subsequently used in perithecium formation on stalk tissue. These results indicate that extensive colonization of plant tissue prior to harvest is essential for subsequent sporulation on crop residues and, thus, has important implications for inoculum reduction.",
author = "Guenther, {John C.} and Hallen-Adams, {Heather E.} and Heike B{\"u}cking and Yair Shachar-Hill and Frances Trail",
year = "2009",
month = "12",
day = "1",
doi = "10.1094/MPMI-22-12-1492",
language = "English (US)",
volume = "22",
pages = "1492--1503",
journal = "Molecular Plant-Microbe Interactions",
issn = "0894-0282",
publisher = "American Phytopathological Society",
number = "12",

}

TY - JOUR

T1 - Triacylglyceride metabolism by Fusarium graminearum during colonization and sexual development on wheat

AU - Guenther, John C.

AU - Hallen-Adams, Heather E.

AU - Bücking, Heike

AU - Shachar-Hill, Yair

AU - Trail, Frances

PY - 2009/12/1

Y1 - 2009/12/1

N2 - Fusarium graminearum, a devastating pathogen of small grains, overwinters on crop residues and produces ephemeral perithecia. Accumulation of lipids in overwintering hyphae would provide reserves for overwinter survival and perithecium development. Fatty acid composition of cultures during perithecium development indicated a drop in neutral lipid levels during development but little change in fatty acid composition across stages. Microscopic examination of cultures early in sexual development revealed hyphal cells engorged with lipid bodies. In comparison, vegetative hyphae contained few lipid bodies. Microarray analysis was performed on wheat stems at stages of colonization through perithecium development. Gene expression analysis during stages of perithecium development both in planta and in vitro (previously published) supports the view that lipid biosynthesis occurs during early stages of wheat colonization leading to sexual development and that lipid oxidation occurs as perithecia are developing. Analysis of gene expression during the stages of wheat stem colonization also revealed sets of genes unique to these stages. These results support the view that lipids accumulate in hyphae colonizing wheat stalks and are subsequently used in perithecium formation on stalk tissue. These results indicate that extensive colonization of plant tissue prior to harvest is essential for subsequent sporulation on crop residues and, thus, has important implications for inoculum reduction.

AB - Fusarium graminearum, a devastating pathogen of small grains, overwinters on crop residues and produces ephemeral perithecia. Accumulation of lipids in overwintering hyphae would provide reserves for overwinter survival and perithecium development. Fatty acid composition of cultures during perithecium development indicated a drop in neutral lipid levels during development but little change in fatty acid composition across stages. Microscopic examination of cultures early in sexual development revealed hyphal cells engorged with lipid bodies. In comparison, vegetative hyphae contained few lipid bodies. Microarray analysis was performed on wheat stems at stages of colonization through perithecium development. Gene expression analysis during stages of perithecium development both in planta and in vitro (previously published) supports the view that lipid biosynthesis occurs during early stages of wheat colonization leading to sexual development and that lipid oxidation occurs as perithecia are developing. Analysis of gene expression during the stages of wheat stem colonization also revealed sets of genes unique to these stages. These results support the view that lipids accumulate in hyphae colonizing wheat stalks and are subsequently used in perithecium formation on stalk tissue. These results indicate that extensive colonization of plant tissue prior to harvest is essential for subsequent sporulation on crop residues and, thus, has important implications for inoculum reduction.

UR - http://www.scopus.com/inward/record.url?scp=72949097511&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72949097511&partnerID=8YFLogxK

U2 - 10.1094/MPMI-22-12-1492

DO - 10.1094/MPMI-22-12-1492

M3 - Article

C2 - 19888815

AN - SCOPUS:72949097511

VL - 22

SP - 1492

EP - 1503

JO - Molecular Plant-Microbe Interactions

JF - Molecular Plant-Microbe Interactions

SN - 0894-0282

IS - 12

ER -