Trends and determinants of gastric bacterial colonization of preterm neonates in a NICU setting

Ketki Patel, Kavitha Konduru, Alok K. Patra, Dinesh S. Chandel, Pinaki Panigrahi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Newborn gastrointestinal (GI) tract is considered sterile but rapidly acquires a diverse microbiota from its intimate environment. Early acquisition of a bacterial species in the upper GI tract may play a role in establishing the colonic microbiota. There is paucity of molecular data on the upper GI tract microbiota in preterm neonates. Methods: Gastric aspirates from 22 neonates with an average gestational age 27.7 weeks (±2.8), weighing 973.2 grams (±297.9) admitted to a neonatal intensive care unit were collected prospectively from weeks 1-4 of life. All samples were evaluated for microbiota using 16S rRNA-based Denaturing Gradient Gel Electrophoresis. Bacterial species colonization and its association with maternal and neonatal demographics, and neonatal clinical characteristics were analyzed. Results: Bacteroides spp. was the predominant species in all four weeks. Bifidobacterium spp. colonization was significantly higher in exclusively breast milk fed compared to partially breast milk (PBM) fed neonates in first (p = 0.03) and third (p = 0.03) week of life. Anaerobic bacteria colonization decreased from first through fourth week of life (p = 0.03). Aerobic bacteria colonization was highly dynamic throughout the four week period. Premature rupture of membrane (p = 0.05) and birth outside of study hospital (p = 0.006) influenced the acquisition of bacteria in the first week of life. Birth weight was positively correlated with total number of bacterial species (p = 0.002) and anaerobes (p = 0.004) in PBM-fed neonates during the fourth week of life. H. pylori and Ureaplasma were not detected in any of our samples. Conclusion: Gastric bacterial colonization in preterm neonates is unstable during early weeks of life. Delayed oral feeding and use of antibiotics may be responsible for paucity of bacterial species. Monitoring of the gastric microbiota and concurrent examination of stool microbiota may yield important information on the utility of gastric signature patterns for predicting colon microbiota that may drive GI and immune dysfunctions.

Original languageEnglish (US)
Article numbere0114664
JournalPloS one
Volume10
Issue number7
DOIs
StatePublished - Jul 1 2015

Fingerprint

bacterial colonization
Microbiota
Stomach
neonates
stomach
Bacteria
Human Milk
Aerobic bacteria
breast milk
Intensive care units
Upper Gastrointestinal Tract
gastrointestinal system
Weighing
Electrophoresis
Gels
Association reactions
bacteria
Ureaplasma
Anti-Bacterial Agents
Membranes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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Patel, K., Konduru, K., Patra, A. K., Chandel, D. S., & Panigrahi, P. (2015). Trends and determinants of gastric bacterial colonization of preterm neonates in a NICU setting. PloS one, 10(7), [e0114664]. https://doi.org/10.1371/journal.pone.0114664

Trends and determinants of gastric bacterial colonization of preterm neonates in a NICU setting. / Patel, Ketki; Konduru, Kavitha; Patra, Alok K.; Chandel, Dinesh S.; Panigrahi, Pinaki.

In: PloS one, Vol. 10, No. 7, e0114664, 01.07.2015.

Research output: Contribution to journalArticle

Patel, K, Konduru, K, Patra, AK, Chandel, DS & Panigrahi, P 2015, 'Trends and determinants of gastric bacterial colonization of preterm neonates in a NICU setting', PloS one, vol. 10, no. 7, e0114664. https://doi.org/10.1371/journal.pone.0114664
Patel, Ketki ; Konduru, Kavitha ; Patra, Alok K. ; Chandel, Dinesh S. ; Panigrahi, Pinaki. / Trends and determinants of gastric bacterial colonization of preterm neonates in a NICU setting. In: PloS one. 2015 ; Vol. 10, No. 7.
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abstract = "Background: Newborn gastrointestinal (GI) tract is considered sterile but rapidly acquires a diverse microbiota from its intimate environment. Early acquisition of a bacterial species in the upper GI tract may play a role in establishing the colonic microbiota. There is paucity of molecular data on the upper GI tract microbiota in preterm neonates. Methods: Gastric aspirates from 22 neonates with an average gestational age 27.7 weeks (±2.8), weighing 973.2 grams (±297.9) admitted to a neonatal intensive care unit were collected prospectively from weeks 1-4 of life. All samples were evaluated for microbiota using 16S rRNA-based Denaturing Gradient Gel Electrophoresis. Bacterial species colonization and its association with maternal and neonatal demographics, and neonatal clinical characteristics were analyzed. Results: Bacteroides spp. was the predominant species in all four weeks. Bifidobacterium spp. colonization was significantly higher in exclusively breast milk fed compared to partially breast milk (PBM) fed neonates in first (p = 0.03) and third (p = 0.03) week of life. Anaerobic bacteria colonization decreased from first through fourth week of life (p = 0.03). Aerobic bacteria colonization was highly dynamic throughout the four week period. Premature rupture of membrane (p = 0.05) and birth outside of study hospital (p = 0.006) influenced the acquisition of bacteria in the first week of life. Birth weight was positively correlated with total number of bacterial species (p = 0.002) and anaerobes (p = 0.004) in PBM-fed neonates during the fourth week of life. H. pylori and Ureaplasma were not detected in any of our samples. Conclusion: Gastric bacterial colonization in preterm neonates is unstable during early weeks of life. Delayed oral feeding and use of antibiotics may be responsible for paucity of bacterial species. Monitoring of the gastric microbiota and concurrent examination of stool microbiota may yield important information on the utility of gastric signature patterns for predicting colon microbiota that may drive GI and immune dysfunctions.",
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