Treatment of pea (Pisum sativum L.) protoplasts with DNA-damaging agents induces a 39-kilodalton chloroplast protein immunologically related to Escherichia coli RecA

Heriberto D Cerutti, Hesham Z. Ibrahim, André T. Jagendorf

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Organisms must have efficient mechanisms of DNA repair and recombination to prevent alterations in their genetic information due to DNA damage. There is evidence for DNA repair and recombination in plastids of higher plants, although very little is known at the biochemical level. Many chloroplast proteins are of eubacterial ancestry, suggesting that the same could be true for the components of a DNA repair and recombination system. A 39-kD protein, immunologically related to Escherichia coli RecA, is present in chloroplasts of pea (Pisum safivum L). Bandshift gel assays suggest that it binds single-stranded DNA. Its steady-state level is increased by several DNA-damaging agents. These results are consistent with it being a plastid homolog of E. coli RecA protein, presumably involved in DNA repair and recombination, and with the existence of an SOS-like response in pea leaf cells. Experiments with protein synthesis inhibitors suggest that the 39-kD chloroplast protein is encoded in the nucleus.

Original languageEnglish (US)
Pages (from-to)155-163
Number of pages9
JournalPlant Physiology
Volume102
Issue number1
DOIs
StatePublished - Jan 1 1993

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Chloroplast Proteins
Protoplasts
Peas
DNA repair
DNA Repair
Pisum sativum
Genetic Recombination
protoplasts
peas
chloroplasts
Escherichia coli
Plastids
DNA
plastids
proteins
Rec A Recombinases
Pisum
protein synthesis inhibitors
single-stranded DNA
Protein Synthesis Inhibitors

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Treatment of pea (Pisum sativum L.) protoplasts with DNA-damaging agents induces a 39-kilodalton chloroplast protein immunologically related to Escherichia coli RecA. / Cerutti, Heriberto D; Ibrahim, Hesham Z.; Jagendorf, André T.

In: Plant Physiology, Vol. 102, No. 1, 01.01.1993, p. 155-163.

Research output: Contribution to journalArticle

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