Transvenous intramyocardial cellular delivery increases retention in comparison to intracoronary delivery in a porcine model of acute myocardial infarction

Jon C. George, Jonathan Goldberg, Matthew Joseph, Nasreen Abdulhameed, Joshua Crist, Hiranmoy Das, Vincent J. Pompili

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: Clinical trials using intracoronary (IC) delivery of cells have addressed efficacy but the optimal delivery technique is unknown. Our study aimed to determine whether transvenous intramyocardial (TVIM) approach was advantageous for cellular retention in AMI. Methods: Domestic pigs (n = 4) underwent catheterization with coronary angiography and ventriculography prior to infarction and pre- and post-cells. Pigs underwent 90-minute balloon occlusion of the left anterior descending artery (LAD). After one week they were prepared for IC (n = 2) or TVIM (n = 2) delivery of bone marrow mononuclear cells (MNC) labeled with GFP. IC infusion used an over-the-wire catheter to engage the LAD and balloon inflation to prevent retrograde flow. Venography via the coronary sinus was used for TVIM delivery. The anterior interventricular vein was engaged with a guidewire allowing use of the TransAccess™ catheter that is outfitted with an ultrasound tip for visualization. Animals were sacrificed one hour after delivery and tissue was analyzed. Results: Procedures were performed without complication and monitoring was uneventful. 1 × 108 MNC were isolated from each bone marrow (BM) preparation and 1 × 107 MNC delivered. Ventriculography at one week revealed wall motion abnormalities consistent with an anterior AMI. TVIM and IC delivery revealed mean 452 cells per section and 235 cells per section on average, respectively, in the infarct zone (P = 0.01). Conclusion: We have demonstrated that TVIM approach for cell delivery is feasible and safe. Moreover, this approach may provide an advantage over IC infusion in retention of the cellular product; however, larger studies will be necessary.

Original languageEnglish (US)
Pages (from-to)424-431
Number of pages8
JournalJournal of Interventional Cardiology
Volume21
Issue number5
DOIs
StatePublished - Oct 1 2008

Fingerprint

Swine
Myocardial Infarction
Catheters
Arteries
Balloon Occlusion
Sus scrofa
Coronary Sinus
Phlebography
Economic Inflation
Coronary Angiography
Catheterization
Bone Marrow Cells
Infarction
Veins
Bone Marrow
Clinical Trials

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

Cite this

Transvenous intramyocardial cellular delivery increases retention in comparison to intracoronary delivery in a porcine model of acute myocardial infarction. / George, Jon C.; Goldberg, Jonathan; Joseph, Matthew; Abdulhameed, Nasreen; Crist, Joshua; Das, Hiranmoy; Pompili, Vincent J.

In: Journal of Interventional Cardiology, Vol. 21, No. 5, 01.10.2008, p. 424-431.

Research output: Contribution to journalArticle

George, Jon C. ; Goldberg, Jonathan ; Joseph, Matthew ; Abdulhameed, Nasreen ; Crist, Joshua ; Das, Hiranmoy ; Pompili, Vincent J. / Transvenous intramyocardial cellular delivery increases retention in comparison to intracoronary delivery in a porcine model of acute myocardial infarction. In: Journal of Interventional Cardiology. 2008 ; Vol. 21, No. 5. pp. 424-431.
@article{b33bf1f9cccd42dcaf614c001308d9b2,
title = "Transvenous intramyocardial cellular delivery increases retention in comparison to intracoronary delivery in a porcine model of acute myocardial infarction",
abstract = "Background: Clinical trials using intracoronary (IC) delivery of cells have addressed efficacy but the optimal delivery technique is unknown. Our study aimed to determine whether transvenous intramyocardial (TVIM) approach was advantageous for cellular retention in AMI. Methods: Domestic pigs (n = 4) underwent catheterization with coronary angiography and ventriculography prior to infarction and pre- and post-cells. Pigs underwent 90-minute balloon occlusion of the left anterior descending artery (LAD). After one week they were prepared for IC (n = 2) or TVIM (n = 2) delivery of bone marrow mononuclear cells (MNC) labeled with GFP. IC infusion used an over-the-wire catheter to engage the LAD and balloon inflation to prevent retrograde flow. Venography via the coronary sinus was used for TVIM delivery. The anterior interventricular vein was engaged with a guidewire allowing use of the TransAccess™ catheter that is outfitted with an ultrasound tip for visualization. Animals were sacrificed one hour after delivery and tissue was analyzed. Results: Procedures were performed without complication and monitoring was uneventful. 1 × 108 MNC were isolated from each bone marrow (BM) preparation and 1 × 107 MNC delivered. Ventriculography at one week revealed wall motion abnormalities consistent with an anterior AMI. TVIM and IC delivery revealed mean 452 cells per section and 235 cells per section on average, respectively, in the infarct zone (P = 0.01). Conclusion: We have demonstrated that TVIM approach for cell delivery is feasible and safe. Moreover, this approach may provide an advantage over IC infusion in retention of the cellular product; however, larger studies will be necessary.",
author = "George, {Jon C.} and Jonathan Goldberg and Matthew Joseph and Nasreen Abdulhameed and Joshua Crist and Hiranmoy Das and Pompili, {Vincent J.}",
year = "2008",
month = "10",
day = "1",
doi = "10.1111/j.1540-8183.2008.00390.x",
language = "English (US)",
volume = "21",
pages = "424--431",
journal = "Journal of Interventional Cardiology",
issn = "0896-4327",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Transvenous intramyocardial cellular delivery increases retention in comparison to intracoronary delivery in a porcine model of acute myocardial infarction

AU - George, Jon C.

AU - Goldberg, Jonathan

AU - Joseph, Matthew

AU - Abdulhameed, Nasreen

AU - Crist, Joshua

AU - Das, Hiranmoy

AU - Pompili, Vincent J.

PY - 2008/10/1

Y1 - 2008/10/1

N2 - Background: Clinical trials using intracoronary (IC) delivery of cells have addressed efficacy but the optimal delivery technique is unknown. Our study aimed to determine whether transvenous intramyocardial (TVIM) approach was advantageous for cellular retention in AMI. Methods: Domestic pigs (n = 4) underwent catheterization with coronary angiography and ventriculography prior to infarction and pre- and post-cells. Pigs underwent 90-minute balloon occlusion of the left anterior descending artery (LAD). After one week they were prepared for IC (n = 2) or TVIM (n = 2) delivery of bone marrow mononuclear cells (MNC) labeled with GFP. IC infusion used an over-the-wire catheter to engage the LAD and balloon inflation to prevent retrograde flow. Venography via the coronary sinus was used for TVIM delivery. The anterior interventricular vein was engaged with a guidewire allowing use of the TransAccess™ catheter that is outfitted with an ultrasound tip for visualization. Animals were sacrificed one hour after delivery and tissue was analyzed. Results: Procedures were performed without complication and monitoring was uneventful. 1 × 108 MNC were isolated from each bone marrow (BM) preparation and 1 × 107 MNC delivered. Ventriculography at one week revealed wall motion abnormalities consistent with an anterior AMI. TVIM and IC delivery revealed mean 452 cells per section and 235 cells per section on average, respectively, in the infarct zone (P = 0.01). Conclusion: We have demonstrated that TVIM approach for cell delivery is feasible and safe. Moreover, this approach may provide an advantage over IC infusion in retention of the cellular product; however, larger studies will be necessary.

AB - Background: Clinical trials using intracoronary (IC) delivery of cells have addressed efficacy but the optimal delivery technique is unknown. Our study aimed to determine whether transvenous intramyocardial (TVIM) approach was advantageous for cellular retention in AMI. Methods: Domestic pigs (n = 4) underwent catheterization with coronary angiography and ventriculography prior to infarction and pre- and post-cells. Pigs underwent 90-minute balloon occlusion of the left anterior descending artery (LAD). After one week they were prepared for IC (n = 2) or TVIM (n = 2) delivery of bone marrow mononuclear cells (MNC) labeled with GFP. IC infusion used an over-the-wire catheter to engage the LAD and balloon inflation to prevent retrograde flow. Venography via the coronary sinus was used for TVIM delivery. The anterior interventricular vein was engaged with a guidewire allowing use of the TransAccess™ catheter that is outfitted with an ultrasound tip for visualization. Animals were sacrificed one hour after delivery and tissue was analyzed. Results: Procedures were performed without complication and monitoring was uneventful. 1 × 108 MNC were isolated from each bone marrow (BM) preparation and 1 × 107 MNC delivered. Ventriculography at one week revealed wall motion abnormalities consistent with an anterior AMI. TVIM and IC delivery revealed mean 452 cells per section and 235 cells per section on average, respectively, in the infarct zone (P = 0.01). Conclusion: We have demonstrated that TVIM approach for cell delivery is feasible and safe. Moreover, this approach may provide an advantage over IC infusion in retention of the cellular product; however, larger studies will be necessary.

UR - http://www.scopus.com/inward/record.url?scp=52649143146&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52649143146&partnerID=8YFLogxK

U2 - 10.1111/j.1540-8183.2008.00390.x

DO - 10.1111/j.1540-8183.2008.00390.x

M3 - Article

C2 - 19012733

AN - SCOPUS:52649143146

VL - 21

SP - 424

EP - 431

JO - Journal of Interventional Cardiology

JF - Journal of Interventional Cardiology

SN - 0896-4327

IS - 5

ER -