Transport of long-chain fatty acids in Escherichia coli

Evidence for role of fadL gene product as long-chain fatty acid receptor

W. D. Nunn, R. W. Colburn, Paul N Black

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Transport of long-chain fatty acids (LCFA) across the cytoplasmic membrane of Escherichia coli requires functional fadL and fadD genes. The fadD gene codes for an acyl-CoA synthetase (fatty acid: CoA ligase (AMP forming)) which has broad chain length specificity and is loosely bound to the cytoplasmic membrane. The fadL gene codes for a 43,000-dalton cytoplasmic membrane protein which, acting by an unknown mechanism, is needed specifically for LCFA transport. As a first step to define the role of the fadL gene product, studies were performed to determine if it functions as a LCFA receptor. The LCFA-binding activity was quantitated in intact cells in the absence of LCFA transport by comparing the binding of LCFA in fadD fadL and fadD fadL+ strains. These studies revealed that (1) fadD fadL+ strains bind 6-fold more LCFA than fadD fadL strains; (2) fadD fadL strains harboring a plasmid containing the fadL gene bind 16-fold more LCFA than fadD fadL strains harboring only the plasmid vector; and (3) the fadL-specific LCFA-binding activity is regulated by the fadR gene and catabolite repression. Studies with fadL strains harboring fadL plasmids containing in vitro constructed deletions indicate that mutations which alter the physical properties of the 43,000-dalton fadL gene product also affect fadL gene product-specific LCFA-binding activity. Overall, these studies suggest that one role of the fadL gene product in the LCFA transport process is to sequester LCFA at sites in the cell membrane for transport.

Original languageEnglish (US)
Pages (from-to)167-171
Number of pages5
JournalJournal of Biological Chemistry
Volume261
Issue number1
StatePublished - Jan 1 1986

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Escherichia coli
Fatty Acids
Genes
Cell Membrane
Plasmids
Coenzyme A Ligases
Catabolite Repression
Membranes
Sequence Deletion
Adenosine Monophosphate
Coenzyme A
Cell membranes
Ligases
Chain length
Membrane Proteins
Physical properties

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Transport of long-chain fatty acids in Escherichia coli : Evidence for role of fadL gene product as long-chain fatty acid receptor. / Nunn, W. D.; Colburn, R. W.; Black, Paul N.

In: Journal of Biological Chemistry, Vol. 261, No. 1, 01.01.1986, p. 167-171.

Research output: Contribution to journalArticle

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