Transmissible gastroenteritis virus induced apoptosis in swine testes cell cultures

T. Sirinarumitr, J. P. Kluge, Prem S Paul

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Transmissible gastroenteritis virus (TGEV) is a coronavirus which causes severe gastroenteritis and atrophy of intestinal villous epithelial cells in piglets. However, the mechanism of cell death caused by TGEV is not known. In this study, we report that TGEV induces cell death by apoptosis. TGEV-induced apoptosis was demonstrated by agarose gel electrophoresis, electron microscopy, and terminal deoxytransferase digoxigenin-dUTP nick end labeling (TUNEL). Double labeling experiment confirmed the result from electron microscopy and showed that most of the apoptotic cells were bystander cells as they were negative for TGEV nucleic acids. Results of this study indicate that TGEV induces apoptosis in vitro and that most of the cells undergoing apoptosis are bystander cells, thus amplifying the cytopathic effect of TGEV.

Original languageEnglish (US)
Pages (from-to)2471-2485
Number of pages15
JournalArchives of Virology
Volume143
Issue number12
DOIs
StatePublished - Dec 1 1998

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Transmissible gastroenteritis virus
Testis
Swine
Cell Culture Techniques
Apoptosis
Electron Microscopy
Cell Death
Digoxigenin
Coronavirus
Agar Gel Electrophoresis
In Situ Nick-End Labeling
Gastroenteritis
Nucleic Acids
Atrophy
Epithelial Cells

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Transmissible gastroenteritis virus induced apoptosis in swine testes cell cultures. / Sirinarumitr, T.; Kluge, J. P.; Paul, Prem S.

In: Archives of Virology, Vol. 143, No. 12, 01.12.1998, p. 2471-2485.

Research output: Contribution to journalArticle

Sirinarumitr, T. ; Kluge, J. P. ; Paul, Prem S. / Transmissible gastroenteritis virus induced apoptosis in swine testes cell cultures. In: Archives of Virology. 1998 ; Vol. 143, No. 12. pp. 2471-2485.
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