Transitions in histone acetylation reveal boundaries of three separately regulated neighboring loci

Michael D. Litt, Melanie A Simpson, Félix Recillas-Targa, Marie Noëlle Prioleau, Gary Felsenfeld

Research output: Contribution to journalArticle

318 Citations (Scopus)

Abstract

We have studied developmentally regulated patterns of histone acetylation at high resolution across ∼54 kb of DNA containing three independently regulated but neighboring genetic loci. These include a folate receptor gene, a 16 kb condensed chromatin region, the chicken β-globin domain and an adjacent olfactory receptor gene. Within these regions the relative levels of acetylation appear to fall into three classes. The condensed chromatin region maintains the lowest acetylation at every developmental stage. Genes that are inactive show similarly low levels, but activation results in a dramatic increase in acetylation. The highest levels of acetylation are seen at regulatory sites upstream of the genes. These patterns imply the action of more than one class of acetylation. Notably, there is a very strong constitutive focus of hyperacetylation at the 5′ insulator element separating the globin locus from the folate receptor region, which suggests that this insulator element may harbor a high concentration of histone acetylases.

Original languageEnglish (US)
Pages (from-to)2224-2235
Number of pages12
JournalEMBO Journal
Volume20
Issue number9
DOIs
StatePublished - May 1 2001

Fingerprint

Acetylation
Histones
Genes
Insulator Elements
Globins
Folic Acid
Chromatin
Odorant Receptors
Histone Acetyltransferases
Genetic Loci
Ports and harbors
Chickens
Chemical activation
DNA

Keywords

  • Acetylation
  • Boundaries
  • Chromatin domains
  • Folate receptor
  • Globin genes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Litt, M. D., Simpson, M. A., Recillas-Targa, F., Prioleau, M. N., & Felsenfeld, G. (2001). Transitions in histone acetylation reveal boundaries of three separately regulated neighboring loci. EMBO Journal, 20(9), 2224-2235. https://doi.org/10.1093/emboj/20.9.2224

Transitions in histone acetylation reveal boundaries of three separately regulated neighboring loci. / Litt, Michael D.; Simpson, Melanie A; Recillas-Targa, Félix; Prioleau, Marie Noëlle; Felsenfeld, Gary.

In: EMBO Journal, Vol. 20, No. 9, 01.05.2001, p. 2224-2235.

Research output: Contribution to journalArticle

Litt, MD, Simpson, MA, Recillas-Targa, F, Prioleau, MN & Felsenfeld, G 2001, 'Transitions in histone acetylation reveal boundaries of three separately regulated neighboring loci', EMBO Journal, vol. 20, no. 9, pp. 2224-2235. https://doi.org/10.1093/emboj/20.9.2224
Litt, Michael D. ; Simpson, Melanie A ; Recillas-Targa, Félix ; Prioleau, Marie Noëlle ; Felsenfeld, Gary. / Transitions in histone acetylation reveal boundaries of three separately regulated neighboring loci. In: EMBO Journal. 2001 ; Vol. 20, No. 9. pp. 2224-2235.
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