Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation

D. D. Songstad, F. G. Halaka, D. L. DeBoer, C. L. Armstrong, M. A.W. Hinchee, C. G. Ford-Santino, S. M. Brown, Michael E Fromm, R. B. Horsch

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Electroporation was used for the delivery and subsequent expression of GUS and anthocyanin reporter genes into intact maize immature embryos. The optimal conditions consisted of culturing immature embryos for 4 days on N6 1-100-25-Ag medium prior to electroporation (375 V/cm; 960 μF capacitance) in EPR buffer containing DNA and 0.07 M sodium glutamate at room temperature (22°C) after a 10 min heat shock at 37°C. Under these conditions, over 40 spots of GUS transient activity were observed per immature embryo. Transient gene expression after electroporation was further demonstrated using an anthocyanin construct, which is specific for expression in plant cells.

Original languageEnglish (US)
Pages (from-to)195-201
Number of pages7
JournalPlant Cell, Tissue and Organ Culture
Volume33
Issue number2
DOIs
StatePublished - May 1 1993

Fingerprint

Electroporation
electroporation
Anthocyanins
Zea mays
anthocyanins
Embryonic Structures
immatures
corn
monosodium glutamate
Sodium Glutamate
capacitance
Plant Cells
Reporter Genes
reporter genes
heat stress
Shock
ambient temperature
Buffers
buffers
Hot Temperature

Keywords

  • DNA delivery
  • Zea mays
  • electroporation electrolyte
  • heat shock

ASJC Scopus subject areas

  • Horticulture

Cite this

Songstad, D. D., Halaka, F. G., DeBoer, D. L., Armstrong, C. L., Hinchee, M. A. W., Ford-Santino, C. G., ... Horsch, R. B. (1993). Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation. Plant Cell, Tissue and Organ Culture, 33(2), 195-201. https://doi.org/10.1007/BF01983234

Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation. / Songstad, D. D.; Halaka, F. G.; DeBoer, D. L.; Armstrong, C. L.; Hinchee, M. A.W.; Ford-Santino, C. G.; Brown, S. M.; Fromm, Michael E; Horsch, R. B.

In: Plant Cell, Tissue and Organ Culture, Vol. 33, No. 2, 01.05.1993, p. 195-201.

Research output: Contribution to journalArticle

Songstad, DD, Halaka, FG, DeBoer, DL, Armstrong, CL, Hinchee, MAW, Ford-Santino, CG, Brown, SM, Fromm, ME & Horsch, RB 1993, 'Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation', Plant Cell, Tissue and Organ Culture, vol. 33, no. 2, pp. 195-201. https://doi.org/10.1007/BF01983234
Songstad, D. D. ; Halaka, F. G. ; DeBoer, D. L. ; Armstrong, C. L. ; Hinchee, M. A.W. ; Ford-Santino, C. G. ; Brown, S. M. ; Fromm, Michael E ; Horsch, R. B. / Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation. In: Plant Cell, Tissue and Organ Culture. 1993 ; Vol. 33, No. 2. pp. 195-201.
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