Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis

Bin Yu, Elisabeth J. Chapman, Zhiyong Yang, James C. Carrington, Xuemei Chen

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

HEN1-dependent methylation of the 3′-terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC-Pro, p21 and p19) inhibit miRNA methylation. These suppressors have distinct effects on different miRNAs. We also show that miRNA* is methylated in vivo in a suppressor-sensitive manner, suggesting that the viral proteins interfere with miRNA/miRNA* duplexes. p19 and p21 bind both methylated and unmethylated miRNA/miRNA* duplexes in vivo. These findings suggest miRNA/miRNA* as the in vivo substrates for the HEN1 miRNA methyltransferase and raise intriguing possibilities regarding the cellular location of miRNA methylation.

Original languageEnglish (US)
Pages (from-to)3117-3120
Number of pages4
JournalFEBS Letters
Volume580
Issue number13
DOIs
StatePublished - May 29 2006

Fingerprint

Methylation
Viral RNA
RNA Interference
MicroRNAs
Arabidopsis
Methyltransferases
Viral Proteins
Nucleotides

Keywords

  • HEN1
  • Methylation
  • Viral RNA silencing suppressor
  • microRNA/microRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis. / Yu, Bin; Chapman, Elisabeth J.; Yang, Zhiyong; Carrington, James C.; Chen, Xuemei.

In: FEBS Letters, Vol. 580, No. 13, 29.05.2006, p. 3117-3120.

Research output: Contribution to journalArticle

Yu, Bin ; Chapman, Elisabeth J. ; Yang, Zhiyong ; Carrington, James C. ; Chen, Xuemei. / Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis. In: FEBS Letters. 2006 ; Vol. 580, No. 13. pp. 3117-3120.
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