Transgenic mouse model for human gastric carcinoma

K. Koike, Steven Heye Hinrichs, K. J. Isselbacher, G. Jay

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Abstract

To understand the pathogenesis that may be induced by human adenovirus type 12 (Ad12), we have generated transgenic mice carrying the Ad12 early region 1 under control of the mouse mammary tumor virus long terminal repeat. Eleven of 11 male founder mice, but only 2 of 12 females, died between 3 to 4 mo of age. Death was associated with presence of tumors at or near the squamocolumnar junction of the stomach. Microscopically, these multifocal tumors appeared to arise from hyperplastic epithelium and showed features consistent with adenocarcinoma or adenosquamous carcinoma. High levels of expression of both the Ad12 E1A and E1B genes were seen in the tumor-bearing stomach. Various levels of expression were also detected in other tissues, although the stomach was the only organ with detectable pathology. These observations suggest an organ-specific action of the Ad12 early region 1 gene products. This transgenic mouse model provides an experimental system for studying the development of human carcinomas at sites of transition from squamous to columnar epithelium.

Original languageEnglish (US)
Pages (from-to)5615-5619
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number14
DOIs
StatePublished - Jan 1 1989

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Adenoviridae
Transgenic Mice
Stomach
Carcinoma
Epithelium
Adenosquamous Carcinoma
Mouse mammary tumor virus
Human Adenoviruses
Neoplasms
Terminal Repeat Sequences
Human Development
Genes
Adenocarcinoma
Theoretical Models
Pathology

ASJC Scopus subject areas

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Transgenic mouse model for human gastric carcinoma. / Koike, K.; Hinrichs, Steven Heye; Isselbacher, K. J.; Jay, G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 14, 01.01.1989, p. 5615-5619.

Research output: Contribution to journalArticle

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