Transgenic mice expressing IFN-γ in pancreatic β-cells are resistant to streptozotocin-induced diabetes

D. Gu, M. Arnush, S. P. Sawyer, Nora E Sarvetnick

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In 28 adult Ins-IFN-γ transgenic mice, injection of high doses of streptozotocin (STZ; first injection, 300 μg/g body weight; second injection, 200 μg/g body weight 4 h later) failed to induce severe hyperglycemia. To the contrary, 28 BALB/c mice developed diabetes mellitus after identical injections of STZ. Because the STZ-induced islet damage was partially inhibited in Ins-IFN-γ transgenic mice, their glycemia levels became normal 4 days after STZ administration. Both transgenic and BALB/c mice lost weight after receiving STZ, but the body weights of transgenic mice then returned to pretreatment levels in a nearly parallel manner with the glycemia. Immunolabeling with insulin identified an unusual spreading pattern of insulin immunoreactivity. Ultrastructural observations confirmed that β- cell necrosis and degranulation were more severe in STZ-treated BALB/c than in Ins-IFN-γ transgenic mice. Moreover, regeneration of pancreatic duct cells and islet neogenesis were observed in the transgenic mice. Therefore, after STZ treatment, the Ins-IFN-γ transgenic mice apparently were resistant to the induction of severe diabetes, whereas their BALB/c age-matched counterparts succumbed to the disease.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume269
Issue number6 32-6
StatePublished - Dec 1 1995
Externally publishedYes

Fingerprint

Experimental Diabetes Mellitus
streptozotocin
Streptozocin
Medical problems
Transgenic Mice
diabetes
genetically modified organisms
Insulin
mice
Ducts
Injections
Body Weight
cells
injection
blood glucose
body weight
Cell Degranulation
insulin
Pancreatic Ducts
pancreatic ducts

Keywords

  • pancreas
  • pancreatic ductal cell

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Agricultural and Biological Sciences(all)

Cite this

Transgenic mice expressing IFN-γ in pancreatic β-cells are resistant to streptozotocin-induced diabetes. / Gu, D.; Arnush, M.; Sawyer, S. P.; Sarvetnick, Nora E.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 269, No. 6 32-6, 01.12.1995.

Research output: Contribution to journalArticle

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