Transgenic induction of mitochondrial rearrangements for cytoplasmic male sterility in crop plants

Ajay Pal S. Sandhu, Ricardo V. Abdelnoor, Sally Mackenzie

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Stability of the mitochondrial genome is controlled by nuclear loci. In plants, nuclear genes suppress mitochondrial DNA rearrangements during development. One nuclear gene involved in this process is Msh1. Msh1 appears to be involved in the suppression of illegitimate recombination in plant mitochondria. To test the hypothesis that Msh1 disruption leads to the type of mitochondrial DNA rearrangements associated with naturally occurring cytoplasmic male sterility in plants, a transgenic approach for RNAi was used to modulate expression of Msh1 in tobacco and tomato. In both species, these experiments resulted in reproducible mitochondrial DNA rearrangements and a condition of male (pollen) sterility. The male sterility was, in each case, heritable, associated with normal female fertility, and apparently maternal in its inheritance. Segregation of the transgene did not reverse the male sterile phenotype, producing stable, nontransganic male sterility. The reproducible transgenic induction of mitochondrial rearrangements in plants is unprecedented, providing a means to develop novel cytoplasmic male sterile lines for release as non-GMO or transgenic materials.

Original languageEnglish (US)
Pages (from-to)1766-1770
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number6
DOIs
StatePublished - Feb 6 2007

Fingerprint

Male Infertility
Gene Rearrangement
Mitochondrial DNA
Plant Genes
Mitochondrial Genome
Genetically Modified Plants
Lycopersicon esculentum
RNA Interference
Pollen
Transgenes
Genetic Recombination
Tobacco
Fertility
Mitochondria
Mothers
Phenotype
Genes

Keywords

  • MutS homolog
  • Nonhomologous recombination
  • RNA interference
  • Tobacco
  • Tomato

ASJC Scopus subject areas

  • General

Cite this

Transgenic induction of mitochondrial rearrangements for cytoplasmic male sterility in crop plants. / Sandhu, Ajay Pal S.; Abdelnoor, Ricardo V.; Mackenzie, Sally.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 6, 06.02.2007, p. 1766-1770.

Research output: Contribution to journalArticle

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