Transgene and transposon silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA helicase

D. Wu-Scharf, B. R. Jeong, C. Zhang, H. Cerutti

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families. Mut6 encodes a protein that is highly homologous to RNA helicases of the DEAH-box family. This protein is necessary for the degradation of certain aberrant RNAs, such as improperly processed transcripts, which are often produced by transposons and some transgenes.

Original languageEnglish (US)
Pages (from-to)1159-1162
Number of pages4
JournalScience
Volume290
Issue number5494
DOIs
StatePublished - Nov 10 2000

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RNA Helicases
Chlamydomonas reinhardtii
RNA Interference
Transgenes
Chlorophyta
Proteins
RNA
Genes

ASJC Scopus subject areas

  • General

Cite this

Transgene and transposon silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA helicase. / Wu-Scharf, D.; Jeong, B. R.; Zhang, C.; Cerutti, H.

In: Science, Vol. 290, No. 5494, 10.11.2000, p. 1159-1162.

Research output: Contribution to journalArticle

Wu-Scharf, D. ; Jeong, B. R. ; Zhang, C. ; Cerutti, H. / Transgene and transposon silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA helicase. In: Science. 2000 ; Vol. 290, No. 5494. pp. 1159-1162.
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