Transforming growth factors from human colonic carcinoma cells induced molecular alterations in untransformed mouse AKR embryo fibroblasts

Subhas Chakrabarty, Michael G. Brattain

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Over 700 polypeptide spots could be detected by two-dimensional electrophoretic analyses of membranes prepared from the murine AKR fibroblastic cells. Out of this abundance of polypeptides, only 9 polypeptide spots were found to be differentially expressed between the untransformed AKR-2B cells and their methylcholanthrene-transformed counterparts, the AKR-MCA cells. Treatment of the untransformed AKR-2B cells with transforming growth factors, prepared from the serum-free conditioned medium of HCT 116 MOSER human colonic carcinoma cells, induced the altered expression of 6 of these polypeptides which paralleled the electrophoretic profile of their permanently transformed counterparts, the AKR-MCA cells.

Original languageEnglish (US)
Pages (from-to)891-895
Number of pages5
JournalCell Biology International Reports
Issue number11
Publication statusPublished - Nov 1986


ASJC Scopus subject areas

  • Cell Biology

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