Transforming growth factor beta (TGF-B) stimulation of angiogenesis: an electron microscopic study.

G. D. Phillips, R. A. Whitehead, A. M. Stone, M. W. Ruebel, M. L. Goodkin, D. R. Knighton

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The object of this study was to examine the initiation and pattern of corneal angiogenesis stimulated by transforming growth factor beta (TGF-B) using scanning electron microscopy (SEM) of vascular corrosion casts and transmission electron microscopy (TEM). Fifty nanograms of TGF-B was combined with the slow release polymer Hydron and implanted in the rabbit cornea as an assay for angiogenic activity. Two and 7 days after implantation, the corneas were removed for TEM. At the same time intervals the limbal vasculature was filled with Mercox, an acrylic monomer. After the Mercox hardened, the tissue was digested with alternating immersions in 40% KOH and distilled water. The resulting casts were air-dried, sputter coated with gold and viewed with the scanning electron microscope. TEM revealed that 2 days after implantation, TGF-B elicited an influx of a vast number of inflammatory cells, which at two days were predominantly neutrophils. By 7 days the inflammation had subsided and typical capillaries were seen between the collagenous laminae of the normally avascular corneal stroma. SEM of vascular corrosion casts showed evidence of margination/diapedesis of leukocytes from the limbal venules 2 days post-implantation. In addition, the casts from the 7 day time point showed that new vessels arose solely from limbal venules.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalJournal of submicroscopic cytology and pathology
Volume25
Issue number2
StatePublished - Apr 1993

Fingerprint

Transmission Electron Microscopy
Transforming Growth Factor beta
Corrosion
Venules
Electrons
Electron Scanning Microscopy
Cornea
Blood Vessels
Corneal Neovascularization
Transendothelial and Transepithelial Migration
Corneal Stroma
Immersion
Gold
Polymers
Neutrophils
Leukocytes
Cell Count
Air
Rabbits
Inflammation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Phillips, G. D., Whitehead, R. A., Stone, A. M., Ruebel, M. W., Goodkin, M. L., & Knighton, D. R. (1993). Transforming growth factor beta (TGF-B) stimulation of angiogenesis: an electron microscopic study. Journal of submicroscopic cytology and pathology, 25(2), 149-155.

Transforming growth factor beta (TGF-B) stimulation of angiogenesis : an electron microscopic study. / Phillips, G. D.; Whitehead, R. A.; Stone, A. M.; Ruebel, M. W.; Goodkin, M. L.; Knighton, D. R.

In: Journal of submicroscopic cytology and pathology, Vol. 25, No. 2, 04.1993, p. 149-155.

Research output: Contribution to journalArticle

Phillips, GD, Whitehead, RA, Stone, AM, Ruebel, MW, Goodkin, ML & Knighton, DR 1993, 'Transforming growth factor beta (TGF-B) stimulation of angiogenesis: an electron microscopic study.', Journal of submicroscopic cytology and pathology, vol. 25, no. 2, pp. 149-155.
Phillips GD, Whitehead RA, Stone AM, Ruebel MW, Goodkin ML, Knighton DR. Transforming growth factor beta (TGF-B) stimulation of angiogenesis: an electron microscopic study. Journal of submicroscopic cytology and pathology. 1993 Apr;25(2):149-155.
Phillips, G. D. ; Whitehead, R. A. ; Stone, A. M. ; Ruebel, M. W. ; Goodkin, M. L. ; Knighton, D. R. / Transforming growth factor beta (TGF-B) stimulation of angiogenesis : an electron microscopic study. In: Journal of submicroscopic cytology and pathology. 1993 ; Vol. 25, No. 2. pp. 149-155.
@article{c3c044dba524473e91fed821b42cf2c8,
title = "Transforming growth factor beta (TGF-B) stimulation of angiogenesis: an electron microscopic study.",
abstract = "The object of this study was to examine the initiation and pattern of corneal angiogenesis stimulated by transforming growth factor beta (TGF-B) using scanning electron microscopy (SEM) of vascular corrosion casts and transmission electron microscopy (TEM). Fifty nanograms of TGF-B was combined with the slow release polymer Hydron and implanted in the rabbit cornea as an assay for angiogenic activity. Two and 7 days after implantation, the corneas were removed for TEM. At the same time intervals the limbal vasculature was filled with Mercox, an acrylic monomer. After the Mercox hardened, the tissue was digested with alternating immersions in 40{\%} KOH and distilled water. The resulting casts were air-dried, sputter coated with gold and viewed with the scanning electron microscope. TEM revealed that 2 days after implantation, TGF-B elicited an influx of a vast number of inflammatory cells, which at two days were predominantly neutrophils. By 7 days the inflammation had subsided and typical capillaries were seen between the collagenous laminae of the normally avascular corneal stroma. SEM of vascular corrosion casts showed evidence of margination/diapedesis of leukocytes from the limbal venules 2 days post-implantation. In addition, the casts from the 7 day time point showed that new vessels arose solely from limbal venules.",
author = "Phillips, {G. D.} and Whitehead, {R. A.} and Stone, {A. M.} and Ruebel, {M. W.} and Goodkin, {M. L.} and Knighton, {D. R.}",
year = "1993",
month = "4",
language = "English (US)",
volume = "25",
pages = "149--155",
journal = "Journal of Submicroscopic Cytology and Pathology",
issn = "1122-9497",
publisher = "N I E Nuova Immagine Editrice",
number = "2",

}

TY - JOUR

T1 - Transforming growth factor beta (TGF-B) stimulation of angiogenesis

T2 - an electron microscopic study.

AU - Phillips, G. D.

AU - Whitehead, R. A.

AU - Stone, A. M.

AU - Ruebel, M. W.

AU - Goodkin, M. L.

AU - Knighton, D. R.

PY - 1993/4

Y1 - 1993/4

N2 - The object of this study was to examine the initiation and pattern of corneal angiogenesis stimulated by transforming growth factor beta (TGF-B) using scanning electron microscopy (SEM) of vascular corrosion casts and transmission electron microscopy (TEM). Fifty nanograms of TGF-B was combined with the slow release polymer Hydron and implanted in the rabbit cornea as an assay for angiogenic activity. Two and 7 days after implantation, the corneas were removed for TEM. At the same time intervals the limbal vasculature was filled with Mercox, an acrylic monomer. After the Mercox hardened, the tissue was digested with alternating immersions in 40% KOH and distilled water. The resulting casts were air-dried, sputter coated with gold and viewed with the scanning electron microscope. TEM revealed that 2 days after implantation, TGF-B elicited an influx of a vast number of inflammatory cells, which at two days were predominantly neutrophils. By 7 days the inflammation had subsided and typical capillaries were seen between the collagenous laminae of the normally avascular corneal stroma. SEM of vascular corrosion casts showed evidence of margination/diapedesis of leukocytes from the limbal venules 2 days post-implantation. In addition, the casts from the 7 day time point showed that new vessels arose solely from limbal venules.

AB - The object of this study was to examine the initiation and pattern of corneal angiogenesis stimulated by transforming growth factor beta (TGF-B) using scanning electron microscopy (SEM) of vascular corrosion casts and transmission electron microscopy (TEM). Fifty nanograms of TGF-B was combined with the slow release polymer Hydron and implanted in the rabbit cornea as an assay for angiogenic activity. Two and 7 days after implantation, the corneas were removed for TEM. At the same time intervals the limbal vasculature was filled with Mercox, an acrylic monomer. After the Mercox hardened, the tissue was digested with alternating immersions in 40% KOH and distilled water. The resulting casts were air-dried, sputter coated with gold and viewed with the scanning electron microscope. TEM revealed that 2 days after implantation, TGF-B elicited an influx of a vast number of inflammatory cells, which at two days were predominantly neutrophils. By 7 days the inflammation had subsided and typical capillaries were seen between the collagenous laminae of the normally avascular corneal stroma. SEM of vascular corrosion casts showed evidence of margination/diapedesis of leukocytes from the limbal venules 2 days post-implantation. In addition, the casts from the 7 day time point showed that new vessels arose solely from limbal venules.

UR - http://www.scopus.com/inward/record.url?scp=0027582106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027582106&partnerID=8YFLogxK

M3 - Article

C2 - 7686811

AN - SCOPUS:0027582106

VL - 25

SP - 149

EP - 155

JO - Journal of Submicroscopic Cytology and Pathology

JF - Journal of Submicroscopic Cytology and Pathology

SN - 1122-9497

IS - 2

ER -