Transforming growth factor-beta receptor type ii expression in the hamster ovary

Cellular site(s), biochemical properties, and hormonal regulation

Shyamal K Roy, A. R. Kole

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The hormonal regulation of transforming growth factor-beta (TGF beta) receptor type II (T beta RII) protein expression in the hamster ovary was evaluated by [125I]TGF beta 1 cross-linking, immunolocalization, and immunoprecipitation of T beta RII using receptor-specific antibodies. Granulosa cells of preantral and antral follicles, and interstitial and luteal cells showed strong signal on day 1 at 0900 h; interstitial and luteal staining was maximum. Immunoreactivity in the interstitium fell by day 2 and reappeared on day 4. A sharp reduction of immunostaining occurred after the gonadotropin surge. In hypophysectomized hamsters, FSH induced T beta RII in both granulosa and interstitial cells, but LH was effective only on interstitial cells. Whereas 17 beta-estradiol (17 beta E2) efficiently induced interstitial T beta RII, progesterone severely attenuated E2 induction of receptor protein. Apart from a membrane-associated form of T beta RII, a novel cytosolic form of T beta RII was detected. The cytosolic T beta RII is a glycoprotein with N’-linked oligosaccharides, like its membrane counterpart, possessing serine-threonine kinase activity that is TGF beta sensitive. The membrane-associated T beta RII disappeared on day 2 of the cycle, but reappeared by day 4 in the morning. A good correlation was found with the cytosolic form. Hypophysectomy diminished, whereas FSH, LH, and 17 beta E2 increased both forms of T beta RII; however, LH induction of the cytosolic form was greater than that by FSH. Progesterone prevented T beta RII protein integration to the membrane, but testosterone and dihydrotestosterone were also effective in T beta RII induction in the membrane. A high E2/progesterone ratio was an important determinant in T beta RII induction in the cell membrane. These results provide the first direct evidence for the presence of a functional T beta RII in the ovary. The receptor is a serine/threonine kinase and exists as distinct cytosolic and membrane forms that show a unique relationship during the estrous cycle. The induction of ovarian T beta RII is critically and temporally influenced by gonadotropins and ovarian steroid hormones.

Original languageEnglish (US)
Pages (from-to)4610-4620
Number of pages11
JournalEndocrinology
Volume136
Issue number10
DOIs
StatePublished - Jan 1 1995

Fingerprint

Transforming Growth Factor beta Receptors
Cricetinae
Ovary
Membranes
Progesterone
Granulosa Cells
Protein-Serine-Threonine Kinases
transforming growth factor-beta type II receptor
Gonadotropins
Transforming Growth Factor beta
Estradiol
Beta Subunit Luteinizing Hormone
Luteal Cells
Hypophysectomy
Proteins
Estrous Cycle
Dihydrotestosterone
Corpus Luteum
Oligosaccharides
Immunoprecipitation

ASJC Scopus subject areas

  • Endocrinology

Cite this

Transforming growth factor-beta receptor type ii expression in the hamster ovary : Cellular site(s), biochemical properties, and hormonal regulation. / Roy, Shyamal K; Kole, A. R.

In: Endocrinology, Vol. 136, No. 10, 01.01.1995, p. 4610-4620.

Research output: Contribution to journalArticle

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