Transformation of rat bladder epithelial cells by introduction of a single oncogene

A. M. Mann, M. Stevenson, T. Masui, C. D. Borgeson, Samuel Monroe Cohen

Research output: Contribution to journalArticle

Abstract

Malignant transformation of cells in vitro requires the action of cooperating oncogenes. However, the action of a single activated oncogene, if placed under a strong constitutive promoter, is sufficient to transform in vitro established cells. We have investigated the role of cooperating oncogenes in the transformation of normal rat bladder epithelial cells. Effects of polyoma middle T, v-Ha-ras or activated rat c-Ha-ras, independently or in combination with v-myc on in vitro and in vivo behavior of rat bladder epithelial cells were studied. Introduction of polyoma middle T alone, c-Ha-ras or in some cases v-myc into 'normal' rat bladder epithelial cells was sufficient for full malignant transformation of these cells. In addition, introduction of polyoma middle T, activated c-Ha-ras or v-myc transformed cells into nude mice, resulted in development of highly invasive adenocarcinomas. These results indicate that full malignant transformation of normal rat bladder epithelial cells did not require the action of introduced cooperating oncogenes.

Original languageEnglish (US)
Pages (from-to)65-72
Number of pages8
JournalOncogene Research
Volume6
Issue number1
StatePublished - Jan 1 1991

Fingerprint

Oncogenes
Urinary Bladder
Epithelial Cells
Nude Mice
Adenocarcinoma
In Vitro Techniques

Keywords

  • Bladder cancer
  • In vitro transformation
  • Nude mice
  • Oncogenes

ASJC Scopus subject areas

  • Cancer Research

Cite this

Mann, A. M., Stevenson, M., Masui, T., Borgeson, C. D., & Cohen, S. M. (1991). Transformation of rat bladder epithelial cells by introduction of a single oncogene. Oncogene Research, 6(1), 65-72.

Transformation of rat bladder epithelial cells by introduction of a single oncogene. / Mann, A. M.; Stevenson, M.; Masui, T.; Borgeson, C. D.; Cohen, Samuel Monroe.

In: Oncogene Research, Vol. 6, No. 1, 01.01.1991, p. 65-72.

Research output: Contribution to journalArticle

Mann, AM, Stevenson, M, Masui, T, Borgeson, CD & Cohen, SM 1991, 'Transformation of rat bladder epithelial cells by introduction of a single oncogene', Oncogene Research, vol. 6, no. 1, pp. 65-72.
Mann, A. M. ; Stevenson, M. ; Masui, T. ; Borgeson, C. D. ; Cohen, Samuel Monroe. / Transformation of rat bladder epithelial cells by introduction of a single oncogene. In: Oncogene Research. 1991 ; Vol. 6, No. 1. pp. 65-72.
@article{efb2ea85a8ab4fe698946dfa0e0b65d0,
title = "Transformation of rat bladder epithelial cells by introduction of a single oncogene",
abstract = "Malignant transformation of cells in vitro requires the action of cooperating oncogenes. However, the action of a single activated oncogene, if placed under a strong constitutive promoter, is sufficient to transform in vitro established cells. We have investigated the role of cooperating oncogenes in the transformation of normal rat bladder epithelial cells. Effects of polyoma middle T, v-Ha-ras or activated rat c-Ha-ras, independently or in combination with v-myc on in vitro and in vivo behavior of rat bladder epithelial cells were studied. Introduction of polyoma middle T alone, c-Ha-ras or in some cases v-myc into 'normal' rat bladder epithelial cells was sufficient for full malignant transformation of these cells. In addition, introduction of polyoma middle T, activated c-Ha-ras or v-myc transformed cells into nude mice, resulted in development of highly invasive adenocarcinomas. These results indicate that full malignant transformation of normal rat bladder epithelial cells did not require the action of introduced cooperating oncogenes.",
keywords = "Bladder cancer, In vitro transformation, Nude mice, Oncogenes",
author = "Mann, {A. M.} and M. Stevenson and T. Masui and Borgeson, {C. D.} and Cohen, {Samuel Monroe}",
year = "1991",
month = "1",
day = "1",
language = "English (US)",
volume = "6",
pages = "65--72",
journal = "Oncogene Research",
issn = "0890-6487",
number = "1",

}

TY - JOUR

T1 - Transformation of rat bladder epithelial cells by introduction of a single oncogene

AU - Mann, A. M.

AU - Stevenson, M.

AU - Masui, T.

AU - Borgeson, C. D.

AU - Cohen, Samuel Monroe

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Malignant transformation of cells in vitro requires the action of cooperating oncogenes. However, the action of a single activated oncogene, if placed under a strong constitutive promoter, is sufficient to transform in vitro established cells. We have investigated the role of cooperating oncogenes in the transformation of normal rat bladder epithelial cells. Effects of polyoma middle T, v-Ha-ras or activated rat c-Ha-ras, independently or in combination with v-myc on in vitro and in vivo behavior of rat bladder epithelial cells were studied. Introduction of polyoma middle T alone, c-Ha-ras or in some cases v-myc into 'normal' rat bladder epithelial cells was sufficient for full malignant transformation of these cells. In addition, introduction of polyoma middle T, activated c-Ha-ras or v-myc transformed cells into nude mice, resulted in development of highly invasive adenocarcinomas. These results indicate that full malignant transformation of normal rat bladder epithelial cells did not require the action of introduced cooperating oncogenes.

AB - Malignant transformation of cells in vitro requires the action of cooperating oncogenes. However, the action of a single activated oncogene, if placed under a strong constitutive promoter, is sufficient to transform in vitro established cells. We have investigated the role of cooperating oncogenes in the transformation of normal rat bladder epithelial cells. Effects of polyoma middle T, v-Ha-ras or activated rat c-Ha-ras, independently or in combination with v-myc on in vitro and in vivo behavior of rat bladder epithelial cells were studied. Introduction of polyoma middle T alone, c-Ha-ras or in some cases v-myc into 'normal' rat bladder epithelial cells was sufficient for full malignant transformation of these cells. In addition, introduction of polyoma middle T, activated c-Ha-ras or v-myc transformed cells into nude mice, resulted in development of highly invasive adenocarcinomas. These results indicate that full malignant transformation of normal rat bladder epithelial cells did not require the action of introduced cooperating oncogenes.

KW - Bladder cancer

KW - In vitro transformation

KW - Nude mice

KW - Oncogenes

UR - http://www.scopus.com/inward/record.url?scp=0026086980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026086980&partnerID=8YFLogxK

M3 - Article

C2 - 1847743

AN - SCOPUS:0026086980

VL - 6

SP - 65

EP - 72

JO - Oncogene Research

JF - Oncogene Research

SN - 0890-6487

IS - 1

ER -