Transfection activity of layer-by-layer plasmid DNA/poly(ethylenimine) films deposited on PLGA microparticles

Sandeep Kakade, Devika Soundara Manickam, Hitesh Handa, Guangzhao Mao, David Oupický

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Layer-by-layer (LbL) assemblies of DNA and polycations on the surface of colloidal templates can be used for gene delivery. Plasmid DNA encoding for secreted alkaline phosphatase (SEAP) was used to deposit LbL films with poly(ethylenimine) (PEI) on the surface of polystyrene and poly(lactide-co-glycolide) microparticles. The formation of LBL films was confirmed by zeta potential analysis and fluorescence and atomic force microscopy techniques. The LbL particles were rapidly internalized in a dose-dependent manner by J774.1 murine macrophages. Transfection activity of the LbL particles was evaluated in J774.1 cells using three different doses (5, 10, 25 particle per cell). The levels of SEAP expression increased with increasing dose but were lower than transfection levels mediated by control PEI/DNA polyplexes at corresponding DNA doses. The LbL particles reported here present a promising platform for delivery of DNA to phagocytic cells.

Original languageEnglish (US)
Pages (from-to)44-52
Number of pages9
JournalInternational Journal of Pharmaceutics
Volume365
Issue number1-2
DOIs
StatePublished - Jan 5 2009

Fingerprint

Transfection
Plasmids
DNA
Alkaline Phosphatase
Polyglactin 910
Atomic Force Microscopy
Polystyrenes
Phagocytes
Fluorescence
Macrophages
aziridine
polylactic acid-polyglycolic acid copolymer
Genes

Keywords

  • Layer-by-layer
  • Microparticles
  • PLGA
  • Phagocytosis
  • Plasmid DNA
  • Transfection

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Transfection activity of layer-by-layer plasmid DNA/poly(ethylenimine) films deposited on PLGA microparticles. / Kakade, Sandeep; Manickam, Devika Soundara; Handa, Hitesh; Mao, Guangzhao; Oupický, David.

In: International Journal of Pharmaceutics, Vol. 365, No. 1-2, 05.01.2009, p. 44-52.

Research output: Contribution to journalArticle

Kakade, Sandeep ; Manickam, Devika Soundara ; Handa, Hitesh ; Mao, Guangzhao ; Oupický, David. / Transfection activity of layer-by-layer plasmid DNA/poly(ethylenimine) films deposited on PLGA microparticles. In: International Journal of Pharmaceutics. 2009 ; Vol. 365, No. 1-2. pp. 44-52.
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