Transcripts of the npm-alk fusion gene in anaplastic large cell lymphoma, Hodgkin's disease, and reactive lymphoid lesions

P. G. Elmberger, M. D. Lozano, D. D. Weisenburger, W. Sanger, W. C. Chan

Research output: Contribution to journalArticle

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Abstract

Anaplastic large cell lymphoma (ALCL) and Hodgkin's disease (HD) have some pathologic and immunohistochemical similarities, and a histogenetic relationship between them has been suggested by some investigators. By cytogenetic study, the t(2;5)(p23;q35) translocation appears to be unique for ALCL. The breakpoints of the t(2;5)(p23;q35) have recently been cloned and are reported to involve a novel tyrosine kinase gene, anaplastic lymphoma kinase (alk), on chromosome 2 and the nucleophosmin gene (npm) on chromosome 5. Therefore, we studied the frequency of npm-alk translocation in ALCL using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay. We also studied HD and a variety of reactive lymphoid lesions since there is contradictory information in the literature on the occurrence of the npm-alk rearrangement in HD. We detected npm-alk hybrid mRNA in 8 of 22 cases of ALCL (36%), but none of the 21 cases of HD or the 11 cases with reactive lesions contained amplifiable template. All positive ALCL had the T or indeterminate phenotype and occurred in young adults or children. There was very good correlation between a cytogenetically detectable t(2;5) and a positive signal by RT-PCR. Our results indicate a selective but relatively infrequent association between the t(2;5) and ALCL of T or indeterminate phenotype, not shared with HD or reactive hyperplasia.

Original languageEnglish (US)
Pages (from-to)3517-3521
Number of pages5
JournalBlood
Volume86
Issue number9
StatePublished - Jan 1 1995

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Anaplastic Large-Cell Lymphoma
Gene Fusion
Hodgkin Disease
Fusion reactions
Genes
Polymerase chain reaction
RNA-Directed DNA Polymerase
Chromosomes
Reverse Transcriptase Polymerase Chain Reaction
Phenotype
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 2
Protein-Tyrosine Kinases
Assays
Gene Frequency
Cytogenetics
Hyperplasia
nucleophosmin
anaplastic lymphoma kinase
Association reactions

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Elmberger, P. G., Lozano, M. D., Weisenburger, D. D., Sanger, W., & Chan, W. C. (1995). Transcripts of the npm-alk fusion gene in anaplastic large cell lymphoma, Hodgkin's disease, and reactive lymphoid lesions. Blood, 86(9), 3517-3521.

Transcripts of the npm-alk fusion gene in anaplastic large cell lymphoma, Hodgkin's disease, and reactive lymphoid lesions. / Elmberger, P. G.; Lozano, M. D.; Weisenburger, D. D.; Sanger, W.; Chan, W. C.

In: Blood, Vol. 86, No. 9, 01.01.1995, p. 3517-3521.

Research output: Contribution to journalArticle

Elmberger, PG, Lozano, MD, Weisenburger, DD, Sanger, W & Chan, WC 1995, 'Transcripts of the npm-alk fusion gene in anaplastic large cell lymphoma, Hodgkin's disease, and reactive lymphoid lesions', Blood, vol. 86, no. 9, pp. 3517-3521.
Elmberger PG, Lozano MD, Weisenburger DD, Sanger W, Chan WC. Transcripts of the npm-alk fusion gene in anaplastic large cell lymphoma, Hodgkin's disease, and reactive lymphoid lesions. Blood. 1995 Jan 1;86(9):3517-3521.
Elmberger, P. G. ; Lozano, M. D. ; Weisenburger, D. D. ; Sanger, W. ; Chan, W. C. / Transcripts of the npm-alk fusion gene in anaplastic large cell lymphoma, Hodgkin's disease, and reactive lymphoid lesions. In: Blood. 1995 ; Vol. 86, No. 9. pp. 3517-3521.
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