Transcriptomic comparison of invasive bigheaded carps (Hypophthalmichthys nobilis and Hypophthalmichthys molitrix) and their hybrids

Jun Wang, James T. Lamer, Sarah Gaughan, Michael Wachholtz, Chenghui Wang, Guoqing Lu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix), collectively called bigheaded carps, are invasive species in the Mississippi River Basin (MRB). Interspecific hybridization between bigheaded carps has been considered rare within their native rivers in China; however, it is prevalent in the MRB. We conducted de novo transcriptome analysis of pure and hybrid bigheaded carps and obtained 40,759 to 51,706 transcripts for pure, F1 hybrid, and backcross bigheaded carps. The search against protein databases resulted in 20,336–28,133 annotated transcripts (over 50% of the transcriptome) with over 13,000 transcripts mapped to 23 Gene Ontology biological processes and 127 KEGG metabolic pathways. More transcripts were detected in silver carp than in bighead carp; however, comparable numbers of transcripts were annotated. Transcriptomic variation detected between two F1 hybrids may indicate a potential loss of fitness in hybrids. The neighbor-joining distance tree constructed using over 2,500 one-to-one orthologous sequences suggests transcriptomes could be used to infer the history of introgression and hybridization. Moreover, we detected 24,792 candidate SNPs that can be used to identify different species. The transcriptomes, orthologous sequences, and candidate SNPs obtained in this study should provide further knowledge of interspecific hybridization and introgression.

Original languageEnglish (US)
Pages (from-to)8452-8459
Number of pages8
JournalEcology and Evolution
Volume6
Issue number23
DOIs
StatePublished - Dec 1 2016

Fingerprint

Hypophthalmichthys nobilis
Hypophthalmichthys molitrix
transcriptomics
carp
transcriptome
introgression
silver
river basin
Mississippi River
interspecific hybridization
invasive species
biological processes
fitness
protein
comparison
gene
history
biochemical pathways
river
hybridization

Keywords

  • bigheaded carps
  • hybridization
  • invasion
  • transcriptomes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Nature and Landscape Conservation

Cite this

Transcriptomic comparison of invasive bigheaded carps (Hypophthalmichthys nobilis and Hypophthalmichthys molitrix) and their hybrids. / Wang, Jun; Lamer, James T.; Gaughan, Sarah; Wachholtz, Michael; Wang, Chenghui; Lu, Guoqing.

In: Ecology and Evolution, Vol. 6, No. 23, 01.12.2016, p. 8452-8459.

Research output: Contribution to journalArticle

Wang, Jun ; Lamer, James T. ; Gaughan, Sarah ; Wachholtz, Michael ; Wang, Chenghui ; Lu, Guoqing. / Transcriptomic comparison of invasive bigheaded carps (Hypophthalmichthys nobilis and Hypophthalmichthys molitrix) and their hybrids. In: Ecology and Evolution. 2016 ; Vol. 6, No. 23. pp. 8452-8459.
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