Transcriptome-wide changes in Chlamydomonas reinhardtii gene expression regulated by carbon dioxide and the CO2-concentrating mechanism regulator CIA5/CCM1

Wei Fang, Yaqing Si, Stephen Douglass, David Casero, Sabeeha S. Merchant, Matteo Pellegrini, Istvan Ladunga, Peng Liu, Martin H. Spaldinga

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

We used RNA sequencing to query the Chlamydomonas reinhardtii transcriptome for regulation by CO2 and by the transcription regulator CIA5 (CCM1). Both CO2 and CIA5 are known to play roles in acclimation to low CO2 and in induction of an essential CO2-concentrating mechanism (CCM), but less is known about their interaction and impact on the whole transcriptome. Our comparison of the transcriptome of a wild type versus a cia5 mutant strain under three different CO2 conditions, high CO2 (5%), low CO2 (0.03 to 0.05%), and very low CO2 (<0.02%), provided an entry into global changes in the gene expression patterns occurring in response to the interaction between CO2 and CIA5. We observed a massive impact of CIA5 and CO2 on the transcriptome, affecting almost 25% of all Chlamydomonas genes, and we discovered an array of gene clusters with distinctive expression patterns that provide insight into the regulatory interaction between CIA5 and CO2. Several individual clusters respond primarily to either CIA5 or CO2, providing access to genes regulated by one factor but decoupled from the other. Three distinct clusters clearly associated with CCM-related genes may represent a rich source of candidates for new CCM components, including a small cluster of genes encoding putative inorganic carbon transporters.

Original languageEnglish (US)
Pages (from-to)1876-1893
Number of pages18
JournalPlant Cell
Volume24
Issue number5
DOIs
StatePublished - May 1 2012

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Chlamydomonas reinhardtii
concentrating
Transcriptome
Carbon Dioxide
transcriptome
carbon dioxide
Gene Expression
gene expression
Multigene Family
Genes
RNA Sequence Analysis
Chlamydomonas
Acclimatization
Carbon
multigene family
genes
global change

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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Transcriptome-wide changes in Chlamydomonas reinhardtii gene expression regulated by carbon dioxide and the CO2-concentrating mechanism regulator CIA5/CCM1. / Fang, Wei; Si, Yaqing; Douglass, Stephen; Casero, David; Merchant, Sabeeha S.; Pellegrini, Matteo; Ladunga, Istvan; Liu, Peng; Spaldinga, Martin H.

In: Plant Cell, Vol. 24, No. 5, 01.05.2012, p. 1876-1893.

Research output: Contribution to journalArticle

Fang, W, Si, Y, Douglass, S, Casero, D, Merchant, SS, Pellegrini, M, Ladunga, I, Liu, P & Spaldinga, MH 2012, 'Transcriptome-wide changes in Chlamydomonas reinhardtii gene expression regulated by carbon dioxide and the CO2-concentrating mechanism regulator CIA5/CCM1', Plant Cell, vol. 24, no. 5, pp. 1876-1893. https://doi.org/10.1105/tpc.112.097949
Fang, Wei ; Si, Yaqing ; Douglass, Stephen ; Casero, David ; Merchant, Sabeeha S. ; Pellegrini, Matteo ; Ladunga, Istvan ; Liu, Peng ; Spaldinga, Martin H. / Transcriptome-wide changes in Chlamydomonas reinhardtii gene expression regulated by carbon dioxide and the CO2-concentrating mechanism regulator CIA5/CCM1. In: Plant Cell. 2012 ; Vol. 24, No. 5. pp. 1876-1893.
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