Transcriptional responses of Candida albicans to epithelial and endothelial cells

Hyunsook Park, Yaoping Liu, Norma Solis, Joshua Spotkov, Jessica Hamaker, Jill R Blankenship, Michael R. Yeaman, Aaron P. Mitchell, Haoping Liu, Scott G. Filler

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Candida albicans interacts with oral epithelial cells during oropharyngeal candidiasis and with vascular endothelial cells when it disseminates hematogenously. We set out to identify C. albicans genes that govern interactions with these host cells in vitro. The transcriptional response of C. albicans to the FaDu oral epithelial cell line and primary endothelial cells was determined by microarray analysis. Contact with epithelial cells caused a decrease in transcript levels of genes related to protein synthesis and adhesion, whereas contact with endothelial cells did not significantly influence any specific functional category of genes. Many genes whose transcripts were increased in response to either host cell had not been previously characterized. We constructed mutants with homozygous insertions in 22 of these uncharacterized genes to investigate their function during host-pathogen interaction. By this approach, we found that YCK2, VPSS1, and UEC1 are required for C. albicans to cause normal damage to epithelial cells and resist antimicrobial peptides. YCK2 is also necessary for maintenance of cell polarity. VPS51 is necessary for normal vacuole formation, resistance to multiple stressors, and induction of maximal endothelial cell damage. UEC1 encodes a unique protein that is required for resistance to cell membrane stress. Therefore, some C. albicans genes whose transcripts are increased upon contact with epithelial or endothelial cells are required for the organism to damage these cells and withstand the stresses that it likely encounters during growth in the oropharynx and bloodstream.

Original languageEnglish (US)
Pages (from-to)1498-1510
Number of pages13
JournalEukaryotic Cell
Volume8
Issue number10
DOIs
StatePublished - Oct 1 2009

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Candida albicans
Endothelial Cells
Epithelial Cells
Genes
Host-Pathogen Interactions
Cell Polarity
Oropharynx
Candidiasis
Microarray Analysis
Vacuoles
Proteins
Maintenance
Cell Membrane
Cell Line
Peptides
Growth

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Park, H., Liu, Y., Solis, N., Spotkov, J., Hamaker, J., Blankenship, J. R., ... Filler, S. G. (2009). Transcriptional responses of Candida albicans to epithelial and endothelial cells. Eukaryotic Cell, 8(10), 1498-1510. https://doi.org/10.1128/EC.00165-09

Transcriptional responses of Candida albicans to epithelial and endothelial cells. / Park, Hyunsook; Liu, Yaoping; Solis, Norma; Spotkov, Joshua; Hamaker, Jessica; Blankenship, Jill R; Yeaman, Michael R.; Mitchell, Aaron P.; Liu, Haoping; Filler, Scott G.

In: Eukaryotic Cell, Vol. 8, No. 10, 01.10.2009, p. 1498-1510.

Research output: Contribution to journalArticle

Park, H, Liu, Y, Solis, N, Spotkov, J, Hamaker, J, Blankenship, JR, Yeaman, MR, Mitchell, AP, Liu, H & Filler, SG 2009, 'Transcriptional responses of Candida albicans to epithelial and endothelial cells', Eukaryotic Cell, vol. 8, no. 10, pp. 1498-1510. https://doi.org/10.1128/EC.00165-09
Park, Hyunsook ; Liu, Yaoping ; Solis, Norma ; Spotkov, Joshua ; Hamaker, Jessica ; Blankenship, Jill R ; Yeaman, Michael R. ; Mitchell, Aaron P. ; Liu, Haoping ; Filler, Scott G. / Transcriptional responses of Candida albicans to epithelial and endothelial cells. In: Eukaryotic Cell. 2009 ; Vol. 8, No. 10. pp. 1498-1510.
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