Transcriptional Regulation of the Antioxidant Protein 2 Gene, a Thiol-specific Antioxidant, by Lens Epithelium-derived Growth Factor to Protect Cells from Oxidative Stress

Nigar Fatma, Dhirendra P. Singh, Toshimichi Shinohara, Leo T. Chylack

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Antioxidant protein 2 (AOP2), a member of the newly defined family of thiol-specific antioxidant proteins, has been shown to remove H202 and protect proteins and DNA from oxidative stress. Here we report that LEDGF is one of the regulatory factors for the AOP2 gene. We found that LEDGF bound to the heat shock element and to stress-related elements in the AOP2 promoter. It trans-activated expression of AOP2-CAT in COS-7 cells and lens epithelial cells overexpressing LEDGF. Mutations in the heat shock element and stress-related elements of the AOP2 promoter reduced LEDGF-dependent trans-activation. Lens epithelial cells showed a higher level of AOP2 mRNA in the presence of LEDGF. Cells overexpressing LEDGF exhibited a higher level of AOP2 protein, the level of which was directly related to the increase in cellular protection. Thus, LEDGF, by activating the AOP2 gene, protected and enhanced the survival of cells under oxidative stress.

Original languageEnglish (US)
Pages (from-to)48899-48907
Number of pages9
JournalJournal of Biological Chemistry
Volume276
Issue number52
DOIs
StatePublished - Dec 28 2001

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Oxidative stress
Sulfhydryl Compounds
Oxidative Stress
Antioxidants
Genes
Proteins
Lenses
Shock
Hot Temperature
Epithelial Cells
lens epithelium-derived growth factor
Peroxiredoxins
COS Cells
Cell Survival
Chemical activation
Cells
Messenger RNA
Mutation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Transcriptional Regulation of the Antioxidant Protein 2 Gene, a Thiol-specific Antioxidant, by Lens Epithelium-derived Growth Factor to Protect Cells from Oxidative Stress. / Fatma, Nigar; Singh, Dhirendra P.; Shinohara, Toshimichi; Chylack, Leo T.

In: Journal of Biological Chemistry, Vol. 276, No. 52, 28.12.2001, p. 48899-48907.

Research output: Contribution to journalArticle

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