Transcriptional deregulation of myc inlgH/myc 6;7 translocation carrying rat immunocytomas

Håkan Axelson, Warren S. Pear, Chinmay Kumar Panda, Herve' Bazin, George Klein, Janos Sümegi

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Abstract

We have previously shown that the reciprocal translocation t(6;7) associated with the spontaneous immunocytoma of the Louvain rat (RIC) leads to the juxtaposition of myc to the lgH cluster. In 10 of 14 tumors investigated the breakpoints on the myc carrying chromosome were clustered in a 1.5 kb region 5′ of the intact gene, proximal to the myc promoters. In this paper we describe the effect of the translocation on myc transcription in the RIC system. Run‐on analysis showed transcriptional attenuation in the normal rat myc gene, similar to the situation in mice and humans. The attenuation was almost completely abrogated in the three immunocytomas studied. Sequence analysis of two tumors failed to reveal any structural changes within exon I, as found by others in Burkitt's lymphoma. We also show that the transcriptional initiation of myc mRNA is changed in the RICs. In an established line of rat fibroblasts (Rat‐2), the more distal myc promoter (P2) is the preferred site of initiation. In RIC, however, only 30% of transcripts were initiated from P2. We found that 40% of the transcripts were initiated from PI and 30% from a novel promoter, designated PI a, located between PI and P2.

Original languageEnglish (US)
Pages (from-to)142-148
Number of pages7
JournalGenes, Chromosomes and Cancer
Volume3
Issue number2
DOIs
StatePublished - Mar 1991

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myc Genes
Burkitt Lymphoma
Sequence Analysis
Exons
Neoplasms
Fibroblasts
Chromosomes
Messenger RNA
Genes

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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Transcriptional deregulation of myc inlgH/myc 6;7 translocation carrying rat immunocytomas. / Axelson, Håkan; Pear, Warren S.; Panda, Chinmay Kumar; Bazin, Herve'; Klein, George; Sümegi, Janos.

In: Genes, Chromosomes and Cancer, Vol. 3, No. 2, 03.1991, p. 142-148.

Research output: Contribution to journalArticle

Axelson, H, Pear, WS, Panda, CK, Bazin, H, Klein, G & Sümegi, J 1991, 'Transcriptional deregulation of myc inlgH/myc 6;7 translocation carrying rat immunocytomas', Genes, Chromosomes and Cancer, vol. 3, no. 2, pp. 142-148. https://doi.org/10.1002/gcc.2870030210
Axelson, Håkan ; Pear, Warren S. ; Panda, Chinmay Kumar ; Bazin, Herve' ; Klein, George ; Sümegi, Janos. / Transcriptional deregulation of myc inlgH/myc 6;7 translocation carrying rat immunocytomas. In: Genes, Chromosomes and Cancer. 1991 ; Vol. 3, No. 2. pp. 142-148.
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