Transcriptional control, but not subcellular location, of PGC-1α is altered following exercise in a hot environment

Matthew W. Heesch, Robert J. Shute, Jodi L. Kreiling, Dustin R Slivka

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    The purpose of this study was to determine mitochondrial biogenesisrelated mRNA expression, binding of transcription factors to the peroxisome proliferator-activated receptor- coactivator 1- (PGC-1) promoter, and subcellular location of PGC-1 protein in human skeletal muscle following exercise in a hot environment compared with a room temperature environment. Recreationally trained males (n 11) completed two trials in a temperature-and humiditycontrolled environmental chamber. Each trial consisted of cycling in either a hot (H) or room temperature (C) environment (33 and 20C, respectively) for 1 h at 60% of maximum wattage (Wmax) followed by 3 h of supine recovery at room temperature. Muscle biopsies were taken from the vastus lateralis pre-, post-, and 3 h postexercise. PGC-1 mRNA increased post (P 0.039)-and 3 h postexercise in C (P 0.002). PGC-1, estrogen-related receptor- (ERR), and nuclear respiratory factor 1 (NRF-1) mRNA was all lower in H than C post (P 0.038, P<0.001, and P 0.030, respectively)-and 3 h postexercise (P 0.035, P 0.007, and P<0.001, respectively). Binding of cAMP response element-binding protein (CREB) (P 0.005), myocyte enhancer factor 2 (MEF2) (P 0.047), and FoxO forkhead box class-O1 (FoxO1) (P 0.010) to the promoter region of the PGC-1 gene was lower in H than C. Nuclear PGC-1 protein increased postexercise in both H and C (P 0.029) but was not different between trials (P 0.602). These data indicate that acute exercise in a hot environment blunts expression of mitochondrial biogenesis-related mRNA, due to decreased binding of CREB, MEF2, and FoxO1 to the PGC-1 promoter.

    Original languageEnglish (US)
    Pages (from-to)741-749
    Number of pages9
    JournalJournal of Applied Physiology
    Volume121
    Issue number3
    DOIs
    StatePublished - Sep 1 2016

    Fingerprint

    MEF2 Transcription Factors
    Cyclic AMP Response Element-Binding Protein
    Temperature
    Messenger RNA
    Nuclear Respiratory Factor 1
    Recovery Room
    Peroxisome Proliferator-Activated Receptors
    Quadriceps Muscle
    Organelle Biogenesis
    Genetic Promoter Regions
    Skeletal Muscle
    Proteins
    Transcription Factors
    Exercise
    Biopsy
    Muscles
    Genes

    Keywords

    • Heat stress
    • Mitochondrial biogenesis
    • Skeletal muscle

    ASJC Scopus subject areas

    • Physiology
    • Physiology (medical)

    Cite this

    Transcriptional control, but not subcellular location, of PGC-1α is altered following exercise in a hot environment. / Heesch, Matthew W.; Shute, Robert J.; Kreiling, Jodi L.; Slivka, Dustin R.

    In: Journal of Applied Physiology, Vol. 121, No. 3, 01.09.2016, p. 741-749.

    Research output: Contribution to journalArticle

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    abstract = "The purpose of this study was to determine mitochondrial biogenesisrelated mRNA expression, binding of transcription factors to the peroxisome proliferator-activated receptor- coactivator 1- (PGC-1) promoter, and subcellular location of PGC-1 protein in human skeletal muscle following exercise in a hot environment compared with a room temperature environment. Recreationally trained males (n 11) completed two trials in a temperature-and humiditycontrolled environmental chamber. Each trial consisted of cycling in either a hot (H) or room temperature (C) environment (33 and 20C, respectively) for 1 h at 60{\%} of maximum wattage (Wmax) followed by 3 h of supine recovery at room temperature. Muscle biopsies were taken from the vastus lateralis pre-, post-, and 3 h postexercise. PGC-1 mRNA increased post (P 0.039)-and 3 h postexercise in C (P 0.002). PGC-1, estrogen-related receptor- (ERR), and nuclear respiratory factor 1 (NRF-1) mRNA was all lower in H than C post (P 0.038, P<0.001, and P 0.030, respectively)-and 3 h postexercise (P 0.035, P 0.007, and P<0.001, respectively). Binding of cAMP response element-binding protein (CREB) (P 0.005), myocyte enhancer factor 2 (MEF2) (P 0.047), and FoxO forkhead box class-O1 (FoxO1) (P 0.010) to the promoter region of the PGC-1 gene was lower in H than C. Nuclear PGC-1 protein increased postexercise in both H and C (P 0.029) but was not different between trials (P 0.602). These data indicate that acute exercise in a hot environment blunts expression of mitochondrial biogenesis-related mRNA, due to decreased binding of CREB, MEF2, and FoxO1 to the PGC-1 promoter.",
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    AU - Slivka, Dustin R

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    AB - The purpose of this study was to determine mitochondrial biogenesisrelated mRNA expression, binding of transcription factors to the peroxisome proliferator-activated receptor- coactivator 1- (PGC-1) promoter, and subcellular location of PGC-1 protein in human skeletal muscle following exercise in a hot environment compared with a room temperature environment. Recreationally trained males (n 11) completed two trials in a temperature-and humiditycontrolled environmental chamber. Each trial consisted of cycling in either a hot (H) or room temperature (C) environment (33 and 20C, respectively) for 1 h at 60% of maximum wattage (Wmax) followed by 3 h of supine recovery at room temperature. Muscle biopsies were taken from the vastus lateralis pre-, post-, and 3 h postexercise. PGC-1 mRNA increased post (P 0.039)-and 3 h postexercise in C (P 0.002). PGC-1, estrogen-related receptor- (ERR), and nuclear respiratory factor 1 (NRF-1) mRNA was all lower in H than C post (P 0.038, P<0.001, and P 0.030, respectively)-and 3 h postexercise (P 0.035, P 0.007, and P<0.001, respectively). Binding of cAMP response element-binding protein (CREB) (P 0.005), myocyte enhancer factor 2 (MEF2) (P 0.047), and FoxO forkhead box class-O1 (FoxO1) (P 0.010) to the promoter region of the PGC-1 gene was lower in H than C. Nuclear PGC-1 protein increased postexercise in both H and C (P 0.029) but was not different between trials (P 0.602). These data indicate that acute exercise in a hot environment blunts expression of mitochondrial biogenesis-related mRNA, due to decreased binding of CREB, MEF2, and FoxO1 to the PGC-1 promoter.

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    KW - Skeletal muscle

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