Transamidating enzymes. II. A continuous fluorescent method suited for automating measurements of factor XIII in plasma

L. Lorand, Oksana Lockridge, L. K. Campbell, R. Myhrman, J. Bruner-Lorand

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The covalent incorporation of monodansylcadaverine (N-5-(aminopentyl)-5-dimethylamino-1-naphthalenesulfonamide) into some proteins, catalyzed by a variety of transamidases, is accompanied by marked changes in the fluorescence emission of the dansyl group. In addition to a blue shift, there is an increase in intensity of fluorescence. Various types of caseins, as well as succinylated β-lactoglobulin or lysozyme, can be used as protein acceptors, though neither of the latter two support the reaction in their native form. The continuous rise of fluorescence intensity made it possible to record directly the course of the enzymic transamidating reactions. As such, the procedure is well suited for automation. Furthermore, the assay is applicable to human plasma for the monitoring of fibrin-stabilizing factor (Factor XIII) levels.

Original languageEnglish (US)
Pages (from-to)221-231
Number of pages11
JournalAnalytical Biochemistry
Volume44
Issue number1
DOIs
StatePublished - Jan 1 1971

Fingerprint

Factor XIII
Fluorescence
Plasmas
Enzymes
Plasma (human)
Lactoglobulins
Automation
Muramidase
Caseins
Assays
Proteins
Monitoring
monodansylcadaverine

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Transamidating enzymes. II. A continuous fluorescent method suited for automating measurements of factor XIII in plasma. / Lorand, L.; Lockridge, Oksana; Campbell, L. K.; Myhrman, R.; Bruner-Lorand, J.

In: Analytical Biochemistry, Vol. 44, No. 1, 01.01.1971, p. 221-231.

Research output: Contribution to journalArticle

Lorand, L. ; Lockridge, Oksana ; Campbell, L. K. ; Myhrman, R. ; Bruner-Lorand, J. / Transamidating enzymes. II. A continuous fluorescent method suited for automating measurements of factor XIII in plasma. In: Analytical Biochemistry. 1971 ; Vol. 44, No. 1. pp. 221-231.
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