Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues

S. A. Goff, T. M. Klein, B. A. Roth, Michael E Fromm, K. C. Cone, J. P. Radicella, V. L. Chandler

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

The C1, B and R genes regulating the maize anthocyanin biosynthetic pathway encode tissue-specific regulatory proteins with similarities to transcriptional activators. The C1 and R regulatory genes are usually responsible for pigmentation of seed tissues, and the B-Peru allele of B, but not the B-I allele, can substitute for R function in the seed. In this study, members of the B family of regulatory genes were delivered to intact maize tissues by high velocity microprojectiles. In colorless r aleurones or embryos, the introduction of the B-Peru genomic clone or the expressed cDNAs of B-Peru or B-I resulted in anthocyanin-producing cells. Luciferase produced from the Bronze1 anthocyanin structural gene promoter was induced 100-fold when co-introduced with the expressed B-Peru or B-I cDNAs. This quantitative transactivation assay demonstrates that the proteins encoded by these two B alleles are equally able to transactivate the Bronze1 promoter. Analogous results were obtained using embryogenic callus cells. These observations suggest that one major contribution towards tissue-specific anthocyanine synthesis controlled by the various alleles of the B and R genes is the differential expression of functionally similar proteins.

Original languageEnglish (US)
Pages (from-to)2517-2522
Number of pages6
JournalEMBO Journal
Volume9
Issue number8
StatePublished - Jul 25 1990

Fingerprint

Gene transfer
Peru
Anthocyanins
Regulator Genes
vpr Genes
Transcriptional Activation
Zea mays
Genes
Alleles
Tissue
Seeds
Complementary DNA
Seed
Proteins
Biosynthetic Pathways
Bony Callus
Pigmentation
Luciferases
Embryonic Structures
Clone Cells

Keywords

  • anthocyanine biosynthesis
  • microprojectile bombardment
  • regulatory genes
  • transactivation
  • transcription factors

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Goff, S. A., Klein, T. M., Roth, B. A., Fromm, M. E., Cone, K. C., Radicella, J. P., & Chandler, V. L. (1990). Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues. EMBO Journal, 9(8), 2517-2522.

Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues. / Goff, S. A.; Klein, T. M.; Roth, B. A.; Fromm, Michael E; Cone, K. C.; Radicella, J. P.; Chandler, V. L.

In: EMBO Journal, Vol. 9, No. 8, 25.07.1990, p. 2517-2522.

Research output: Contribution to journalArticle

Goff, SA, Klein, TM, Roth, BA, Fromm, ME, Cone, KC, Radicella, JP & Chandler, VL 1990, 'Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues', EMBO Journal, vol. 9, no. 8, pp. 2517-2522.
Goff SA, Klein TM, Roth BA, Fromm ME, Cone KC, Radicella JP et al. Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues. EMBO Journal. 1990 Jul 25;9(8):2517-2522.
Goff, S. A. ; Klein, T. M. ; Roth, B. A. ; Fromm, Michael E ; Cone, K. C. ; Radicella, J. P. ; Chandler, V. L. / Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues. In: EMBO Journal. 1990 ; Vol. 9, No. 8. pp. 2517-2522.
@article{fecb00e78c2a4df4ad4db076d85e2963,
title = "Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues",
abstract = "The C1, B and R genes regulating the maize anthocyanin biosynthetic pathway encode tissue-specific regulatory proteins with similarities to transcriptional activators. The C1 and R regulatory genes are usually responsible for pigmentation of seed tissues, and the B-Peru allele of B, but not the B-I allele, can substitute for R function in the seed. In this study, members of the B family of regulatory genes were delivered to intact maize tissues by high velocity microprojectiles. In colorless r aleurones or embryos, the introduction of the B-Peru genomic clone or the expressed cDNAs of B-Peru or B-I resulted in anthocyanin-producing cells. Luciferase produced from the Bronze1 anthocyanin structural gene promoter was induced 100-fold when co-introduced with the expressed B-Peru or B-I cDNAs. This quantitative transactivation assay demonstrates that the proteins encoded by these two B alleles are equally able to transactivate the Bronze1 promoter. Analogous results were obtained using embryogenic callus cells. These observations suggest that one major contribution towards tissue-specific anthocyanine synthesis controlled by the various alleles of the B and R genes is the differential expression of functionally similar proteins.",
keywords = "anthocyanine biosynthesis, microprojectile bombardment, regulatory genes, transactivation, transcription factors",
author = "Goff, {S. A.} and Klein, {T. M.} and Roth, {B. A.} and Fromm, {Michael E} and Cone, {K. C.} and Radicella, {J. P.} and Chandler, {V. L.}",
year = "1990",
month = "7",
day = "25",
language = "English (US)",
volume = "9",
pages = "2517--2522",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "8",

}

TY - JOUR

T1 - Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues

AU - Goff, S. A.

AU - Klein, T. M.

AU - Roth, B. A.

AU - Fromm, Michael E

AU - Cone, K. C.

AU - Radicella, J. P.

AU - Chandler, V. L.

PY - 1990/7/25

Y1 - 1990/7/25

N2 - The C1, B and R genes regulating the maize anthocyanin biosynthetic pathway encode tissue-specific regulatory proteins with similarities to transcriptional activators. The C1 and R regulatory genes are usually responsible for pigmentation of seed tissues, and the B-Peru allele of B, but not the B-I allele, can substitute for R function in the seed. In this study, members of the B family of regulatory genes were delivered to intact maize tissues by high velocity microprojectiles. In colorless r aleurones or embryos, the introduction of the B-Peru genomic clone or the expressed cDNAs of B-Peru or B-I resulted in anthocyanin-producing cells. Luciferase produced from the Bronze1 anthocyanin structural gene promoter was induced 100-fold when co-introduced with the expressed B-Peru or B-I cDNAs. This quantitative transactivation assay demonstrates that the proteins encoded by these two B alleles are equally able to transactivate the Bronze1 promoter. Analogous results were obtained using embryogenic callus cells. These observations suggest that one major contribution towards tissue-specific anthocyanine synthesis controlled by the various alleles of the B and R genes is the differential expression of functionally similar proteins.

AB - The C1, B and R genes regulating the maize anthocyanin biosynthetic pathway encode tissue-specific regulatory proteins with similarities to transcriptional activators. The C1 and R regulatory genes are usually responsible for pigmentation of seed tissues, and the B-Peru allele of B, but not the B-I allele, can substitute for R function in the seed. In this study, members of the B family of regulatory genes were delivered to intact maize tissues by high velocity microprojectiles. In colorless r aleurones or embryos, the introduction of the B-Peru genomic clone or the expressed cDNAs of B-Peru or B-I resulted in anthocyanin-producing cells. Luciferase produced from the Bronze1 anthocyanin structural gene promoter was induced 100-fold when co-introduced with the expressed B-Peru or B-I cDNAs. This quantitative transactivation assay demonstrates that the proteins encoded by these two B alleles are equally able to transactivate the Bronze1 promoter. Analogous results were obtained using embryogenic callus cells. These observations suggest that one major contribution towards tissue-specific anthocyanine synthesis controlled by the various alleles of the B and R genes is the differential expression of functionally similar proteins.

KW - anthocyanine biosynthesis

KW - microprojectile bombardment

KW - regulatory genes

KW - transactivation

KW - transcription factors

UR - http://www.scopus.com/inward/record.url?scp=0025307383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025307383&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 2517

EP - 2522

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 8

ER -