Trans-activation of the human immunodeficiency virus long terminal repeat sequence by DNA viruses

H. E. Gendelman, W. Phelps, L. Feigenbaum, J. M. Ostrove, A. Adachi, P. M. Howley, G. Khoury, H. S. Ginsberg, M. A. Martin

Research output: Contribution to journalArticle

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Abstract

To investigate whether DNA viruses can augment gene expression of the human immunodeficiency virus (HIV), cotransfection experiments were carried out in which a recombinant plasmid containing the HIV long terminal repeat (LTR) linked to the chloramphenicol acetyltransferase (CAT) gene was transfected into cultured cells along with plasmids containing DNA from various distinct classes of DNA viruses. Molecular clones containing JC virus, BK virus, lymphotropic papovavirus, bovine papilloma virus, type 1 herpes simplex virus (HSV-1), and varicella-zoster virus sequences increased CAT expression directed by the HIV LTR. Trans-activation of the HIV LTR varied in different cell lines, but in each case the HIV tat gene product elicited the greatest stimulation. Primer-extension assays specific for HIV LTR mRNA revealed increased levels of steady-state RNA following transfection with HIV tat as well as with several of the DNA viruses. Virus-specific RNA expression paralleled the stimulation of CAT activity. More-than-additive effects were observed at both the RNA and protein levels when tat plus type 1 herpes simplex virus DNAs or tat plus JC virus DNAs were transfected into cells with the HIV LTR-CAT plasmid. These data suggest that coinfection of cells by HIV and some DNA viruses can stimulate the expression of HIV.

Original languageEnglish (US)
Pages (from-to)9759-9763
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number24
DOIs
StatePublished - Jan 1 1986

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HIV Long Terminal Repeat
DNA Viruses
Terminal Repeat Sequences
Chloramphenicol O-Acetyltransferase
Human Herpesvirus 1
HIV
JC Virus
Plasmids
DNA
Human Immunodeficiency Virus tat Gene Products
RNA
BK Virus
Human Herpesvirus 3
RNA Viruses
Papilloma
Coinfection
Transfection
Cultured Cells
Clone Cells
Viruses

ASJC Scopus subject areas

  • General

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Trans-activation of the human immunodeficiency virus long terminal repeat sequence by DNA viruses. / Gendelman, H. E.; Phelps, W.; Feigenbaum, L.; Ostrove, J. M.; Adachi, A.; Howley, P. M.; Khoury, G.; Ginsberg, H. S.; Martin, M. A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 24, 01.01.1986, p. 9759-9763.

Research output: Contribution to journalArticle

Gendelman, H. E. ; Phelps, W. ; Feigenbaum, L. ; Ostrove, J. M. ; Adachi, A. ; Howley, P. M. ; Khoury, G. ; Ginsberg, H. S. ; Martin, M. A. / Trans-activation of the human immunodeficiency virus long terminal repeat sequence by DNA viruses. In: Proceedings of the National Academy of Sciences of the United States of America. 1986 ; Vol. 83, No. 24. pp. 9759-9763.
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