Trans-Activation of the HIV Promoter by a cDNA and Its Genomic Clones of Human Herpesvirus-6

Yi Zhou, Charles Wood, Cheow K. Chang, Gao Qian, Bala Chandran

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Human herpesvirus 6 (HHV-6) is a lymphotropic herpesvirus, and in vitro, it can productively infect human CD4+ T cells as HIV-1. Co-infection of T cells by HIV-1 and HHV-6 can lead to both activation of the HIV-1 promoter and acceleration of the cytopathic effects. An HHV-6 (GS) cDNA clone, pCD41, encoding for a 41-kDa nuclear protein was identified and characterized previously (Chang and Balachandran, J. Virol. 65, 2884-2894 and 7085, 1991). Sequence analyses show that this protein has significant hemology with the human cytomegalovirus UL44 gene coding for the ICP36 family of early-late-class phosphoprotein. Using this cDNA as the probe, a 3.8-kb EcoRI genomic fragment encoding the HHV-6(GS)P41 was cloned and designated as pGD41. When cotransfected with the HIV LTR CAT into CV-1 cells, both the pCD41 and pGD41 clones trans-activated the HIV LTR. Sequence analyses of pCD41 indicate that there are two potential open reading frames (ORFs), A and B, which are homologous to the ORFs found in the genomic clone pGD41. Deletion constructs of the pCD41 clone demonstrated that ORF-A was critical for the HIV LTR activation. Deletion analyses of the pCD41 ORF-A and the use of promoter constructs further mapped an internal functional promoter within the pCD41 sequence that can direct the synthesis of the trans -activating protein. By using HIV LTR deletion mutants, the NF-κB binding sites were found to be critical for response to the pCD41 trans -activation.

Original languageEnglish (US)
Article number71129
Pages (from-to)311-322
Number of pages12
JournalVirology
Volume199
Issue number2
DOIs
StatePublished - Mar 1994

Fingerprint

Human Herpesvirus 6
Open Reading Frames
Complementary DNA
Clone Cells
HIV
HIV-1
T-Lymphocytes
Herpesviridae
Phosphoproteins
Protein Sequence Analysis
Nuclear Proteins
Cytomegalovirus
Coinfection
Sequence Analysis
Binding Sites
Genes
Proteins

ASJC Scopus subject areas

  • Virology

Cite this

Trans-Activation of the HIV Promoter by a cDNA and Its Genomic Clones of Human Herpesvirus-6. / Zhou, Yi; Wood, Charles; Chang, Cheow K.; Qian, Gao; Chandran, Bala.

In: Virology, Vol. 199, No. 2, 71129, 03.1994, p. 311-322.

Research output: Contribution to journalArticle

Zhou, Yi ; Wood, Charles ; Chang, Cheow K. ; Qian, Gao ; Chandran, Bala. / Trans-Activation of the HIV Promoter by a cDNA and Its Genomic Clones of Human Herpesvirus-6. In: Virology. 1994 ; Vol. 199, No. 2. pp. 311-322.
@article{476b0aa659654bff861fc05aa2efd460,
title = "Trans-Activation of the HIV Promoter by a cDNA and Its Genomic Clones of Human Herpesvirus-6",
abstract = "Human herpesvirus 6 (HHV-6) is a lymphotropic herpesvirus, and in vitro, it can productively infect human CD4+ T cells as HIV-1. Co-infection of T cells by HIV-1 and HHV-6 can lead to both activation of the HIV-1 promoter and acceleration of the cytopathic effects. An HHV-6 (GS) cDNA clone, pCD41, encoding for a 41-kDa nuclear protein was identified and characterized previously (Chang and Balachandran, J. Virol. 65, 2884-2894 and 7085, 1991). Sequence analyses show that this protein has significant hemology with the human cytomegalovirus UL44 gene coding for the ICP36 family of early-late-class phosphoprotein. Using this cDNA as the probe, a 3.8-kb EcoRI genomic fragment encoding the HHV-6(GS)P41 was cloned and designated as pGD41. When cotransfected with the HIV LTR CAT into CV-1 cells, both the pCD41 and pGD41 clones trans-activated the HIV LTR. Sequence analyses of pCD41 indicate that there are two potential open reading frames (ORFs), A and B, which are homologous to the ORFs found in the genomic clone pGD41. Deletion constructs of the pCD41 clone demonstrated that ORF-A was critical for the HIV LTR activation. Deletion analyses of the pCD41 ORF-A and the use of promoter constructs further mapped an internal functional promoter within the pCD41 sequence that can direct the synthesis of the trans -activating protein. By using HIV LTR deletion mutants, the NF-κB binding sites were found to be critical for response to the pCD41 trans -activation.",
author = "Yi Zhou and Charles Wood and Chang, {Cheow K.} and Gao Qian and Bala Chandran",
year = "1994",
month = "3",
doi = "10.1006/viro.1994.1129",
language = "English (US)",
volume = "199",
pages = "311--322",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Trans-Activation of the HIV Promoter by a cDNA and Its Genomic Clones of Human Herpesvirus-6

AU - Zhou, Yi

AU - Wood, Charles

AU - Chang, Cheow K.

AU - Qian, Gao

AU - Chandran, Bala

PY - 1994/3

Y1 - 1994/3

N2 - Human herpesvirus 6 (HHV-6) is a lymphotropic herpesvirus, and in vitro, it can productively infect human CD4+ T cells as HIV-1. Co-infection of T cells by HIV-1 and HHV-6 can lead to both activation of the HIV-1 promoter and acceleration of the cytopathic effects. An HHV-6 (GS) cDNA clone, pCD41, encoding for a 41-kDa nuclear protein was identified and characterized previously (Chang and Balachandran, J. Virol. 65, 2884-2894 and 7085, 1991). Sequence analyses show that this protein has significant hemology with the human cytomegalovirus UL44 gene coding for the ICP36 family of early-late-class phosphoprotein. Using this cDNA as the probe, a 3.8-kb EcoRI genomic fragment encoding the HHV-6(GS)P41 was cloned and designated as pGD41. When cotransfected with the HIV LTR CAT into CV-1 cells, both the pCD41 and pGD41 clones trans-activated the HIV LTR. Sequence analyses of pCD41 indicate that there are two potential open reading frames (ORFs), A and B, which are homologous to the ORFs found in the genomic clone pGD41. Deletion constructs of the pCD41 clone demonstrated that ORF-A was critical for the HIV LTR activation. Deletion analyses of the pCD41 ORF-A and the use of promoter constructs further mapped an internal functional promoter within the pCD41 sequence that can direct the synthesis of the trans -activating protein. By using HIV LTR deletion mutants, the NF-κB binding sites were found to be critical for response to the pCD41 trans -activation.

AB - Human herpesvirus 6 (HHV-6) is a lymphotropic herpesvirus, and in vitro, it can productively infect human CD4+ T cells as HIV-1. Co-infection of T cells by HIV-1 and HHV-6 can lead to both activation of the HIV-1 promoter and acceleration of the cytopathic effects. An HHV-6 (GS) cDNA clone, pCD41, encoding for a 41-kDa nuclear protein was identified and characterized previously (Chang and Balachandran, J. Virol. 65, 2884-2894 and 7085, 1991). Sequence analyses show that this protein has significant hemology with the human cytomegalovirus UL44 gene coding for the ICP36 family of early-late-class phosphoprotein. Using this cDNA as the probe, a 3.8-kb EcoRI genomic fragment encoding the HHV-6(GS)P41 was cloned and designated as pGD41. When cotransfected with the HIV LTR CAT into CV-1 cells, both the pCD41 and pGD41 clones trans-activated the HIV LTR. Sequence analyses of pCD41 indicate that there are two potential open reading frames (ORFs), A and B, which are homologous to the ORFs found in the genomic clone pGD41. Deletion constructs of the pCD41 clone demonstrated that ORF-A was critical for the HIV LTR activation. Deletion analyses of the pCD41 ORF-A and the use of promoter constructs further mapped an internal functional promoter within the pCD41 sequence that can direct the synthesis of the trans -activating protein. By using HIV LTR deletion mutants, the NF-κB binding sites were found to be critical for response to the pCD41 trans -activation.

UR - http://www.scopus.com/inward/record.url?scp=0028176571&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028176571&partnerID=8YFLogxK

U2 - 10.1006/viro.1994.1129

DO - 10.1006/viro.1994.1129

M3 - Article

C2 - 8122364

AN - SCOPUS:0028176571

VL - 199

SP - 311

EP - 322

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

M1 - 71129

ER -