Towards uncovering the roles of switchgrass peroxidases in plant processes

Aaron J. Saathoff, Teresa Donze, Nathan A. Palmer, Jeff Bradshaw, Tiffany Heng-Moss, Paul Twigg, Christian M. Tobias, Mark Lagrimini, Gautam Sarath

Research output: Contribution to journalReview article

15 Citations (Scopus)

Abstract

Herbaceous perennial plants selected as potential biofuel feedstocks had been understudied at the genomic and functional genomic levels. Recent investments, primarily by the U.S. Department of Energy, have led to the development of a number of molecular resources for bioenergy grasses, such as the partially annotated genome for switchgrass (Panicum virgatum L.), and some related diploid species. In its current version, the switchgrass genome contains 65,878 gene models arising from the A and B genomes of this tetraploid grass. The availability of these gene sequences provides a framework to exploit transcriptomic data obtained from next-generation sequencing platforms to address questions of biological importance. One such question pertains to discovery of genes and proteins important for biotic and abiotic stress responses, and how these components might affect biomass quality and stress response in plants engineered for a specific end purpose. It can be expected that production of switchgrass on marginal lands will expose plants to diverse stresses, including herbivory by insects. Class III plant peroxidases have been implicated in many developmental responses such as lignification and in the adaptive responses of plants to insect feeding. Here, we have analyzed the class III peroxidases encoded by the switchgrass genome, and have mined available transcriptomic datasets to develop a first understanding of the expression profiles of the class III peroxidases in different plant tissues. Lastly, we have identified switchgrass peroxidases that appear to be orthologs of enzymes shown to play key roles in lignification and plant defense responses to hemipterans.

Original languageEnglish (US)
Article number202
JournalFrontiers in Plant Science
Volume4
Issue numberJUN
DOIs
StatePublished - Jun 19 2013

Fingerprint

Panicum virgatum
peroxidases
genome
lignification
transcriptomics
stress response
grasses
genomics
insects
bioenergy
biotic stress
feedstocks
biofuels
abiotic stress
tetraploidy
plant response
plant tissues
Hemiptera
diploidy
herbivores

Keywords

  • Biotic stress
  • Hemiptera
  • Lignin
  • Peroxidases
  • ROS
  • Switchgrass

ASJC Scopus subject areas

  • Plant Science

Cite this

Saathoff, A. J., Donze, T., Palmer, N. A., Bradshaw, J., Heng-Moss, T., Twigg, P., ... Sarath, G. (2013). Towards uncovering the roles of switchgrass peroxidases in plant processes. Frontiers in Plant Science, 4(JUN), [202]. https://doi.org/10.3389/fpls.2013.00202

Towards uncovering the roles of switchgrass peroxidases in plant processes. / Saathoff, Aaron J.; Donze, Teresa; Palmer, Nathan A.; Bradshaw, Jeff; Heng-Moss, Tiffany; Twigg, Paul; Tobias, Christian M.; Lagrimini, Mark; Sarath, Gautam.

In: Frontiers in Plant Science, Vol. 4, No. JUN, 202, 19.06.2013.

Research output: Contribution to journalReview article

Saathoff, AJ, Donze, T, Palmer, NA, Bradshaw, J, Heng-Moss, T, Twigg, P, Tobias, CM, Lagrimini, M & Sarath, G 2013, 'Towards uncovering the roles of switchgrass peroxidases in plant processes', Frontiers in Plant Science, vol. 4, no. JUN, 202. https://doi.org/10.3389/fpls.2013.00202
Saathoff, Aaron J. ; Donze, Teresa ; Palmer, Nathan A. ; Bradshaw, Jeff ; Heng-Moss, Tiffany ; Twigg, Paul ; Tobias, Christian M. ; Lagrimini, Mark ; Sarath, Gautam. / Towards uncovering the roles of switchgrass peroxidases in plant processes. In: Frontiers in Plant Science. 2013 ; Vol. 4, No. JUN.
@article{2f10da00cd814678bdb52e01ff758340,
title = "Towards uncovering the roles of switchgrass peroxidases in plant processes",
abstract = "Herbaceous perennial plants selected as potential biofuel feedstocks had been understudied at the genomic and functional genomic levels. Recent investments, primarily by the U.S. Department of Energy, have led to the development of a number of molecular resources for bioenergy grasses, such as the partially annotated genome for switchgrass (Panicum virgatum L.), and some related diploid species. In its current version, the switchgrass genome contains 65,878 gene models arising from the A and B genomes of this tetraploid grass. The availability of these gene sequences provides a framework to exploit transcriptomic data obtained from next-generation sequencing platforms to address questions of biological importance. One such question pertains to discovery of genes and proteins important for biotic and abiotic stress responses, and how these components might affect biomass quality and stress response in plants engineered for a specific end purpose. It can be expected that production of switchgrass on marginal lands will expose plants to diverse stresses, including herbivory by insects. Class III plant peroxidases have been implicated in many developmental responses such as lignification and in the adaptive responses of plants to insect feeding. Here, we have analyzed the class III peroxidases encoded by the switchgrass genome, and have mined available transcriptomic datasets to develop a first understanding of the expression profiles of the class III peroxidases in different plant tissues. Lastly, we have identified switchgrass peroxidases that appear to be orthologs of enzymes shown to play key roles in lignification and plant defense responses to hemipterans.",
keywords = "Biotic stress, Hemiptera, Lignin, Peroxidases, ROS, Switchgrass",
author = "Saathoff, {Aaron J.} and Teresa Donze and Palmer, {Nathan A.} and Jeff Bradshaw and Tiffany Heng-Moss and Paul Twigg and Tobias, {Christian M.} and Mark Lagrimini and Gautam Sarath",
year = "2013",
month = "6",
day = "19",
doi = "10.3389/fpls.2013.00202",
language = "English (US)",
volume = "4",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media S. A.",
number = "JUN",

}

TY - JOUR

T1 - Towards uncovering the roles of switchgrass peroxidases in plant processes

AU - Saathoff, Aaron J.

AU - Donze, Teresa

AU - Palmer, Nathan A.

AU - Bradshaw, Jeff

AU - Heng-Moss, Tiffany

AU - Twigg, Paul

AU - Tobias, Christian M.

AU - Lagrimini, Mark

AU - Sarath, Gautam

PY - 2013/6/19

Y1 - 2013/6/19

N2 - Herbaceous perennial plants selected as potential biofuel feedstocks had been understudied at the genomic and functional genomic levels. Recent investments, primarily by the U.S. Department of Energy, have led to the development of a number of molecular resources for bioenergy grasses, such as the partially annotated genome for switchgrass (Panicum virgatum L.), and some related diploid species. In its current version, the switchgrass genome contains 65,878 gene models arising from the A and B genomes of this tetraploid grass. The availability of these gene sequences provides a framework to exploit transcriptomic data obtained from next-generation sequencing platforms to address questions of biological importance. One such question pertains to discovery of genes and proteins important for biotic and abiotic stress responses, and how these components might affect biomass quality and stress response in plants engineered for a specific end purpose. It can be expected that production of switchgrass on marginal lands will expose plants to diverse stresses, including herbivory by insects. Class III plant peroxidases have been implicated in many developmental responses such as lignification and in the adaptive responses of plants to insect feeding. Here, we have analyzed the class III peroxidases encoded by the switchgrass genome, and have mined available transcriptomic datasets to develop a first understanding of the expression profiles of the class III peroxidases in different plant tissues. Lastly, we have identified switchgrass peroxidases that appear to be orthologs of enzymes shown to play key roles in lignification and plant defense responses to hemipterans.

AB - Herbaceous perennial plants selected as potential biofuel feedstocks had been understudied at the genomic and functional genomic levels. Recent investments, primarily by the U.S. Department of Energy, have led to the development of a number of molecular resources for bioenergy grasses, such as the partially annotated genome for switchgrass (Panicum virgatum L.), and some related diploid species. In its current version, the switchgrass genome contains 65,878 gene models arising from the A and B genomes of this tetraploid grass. The availability of these gene sequences provides a framework to exploit transcriptomic data obtained from next-generation sequencing platforms to address questions of biological importance. One such question pertains to discovery of genes and proteins important for biotic and abiotic stress responses, and how these components might affect biomass quality and stress response in plants engineered for a specific end purpose. It can be expected that production of switchgrass on marginal lands will expose plants to diverse stresses, including herbivory by insects. Class III plant peroxidases have been implicated in many developmental responses such as lignification and in the adaptive responses of plants to insect feeding. Here, we have analyzed the class III peroxidases encoded by the switchgrass genome, and have mined available transcriptomic datasets to develop a first understanding of the expression profiles of the class III peroxidases in different plant tissues. Lastly, we have identified switchgrass peroxidases that appear to be orthologs of enzymes shown to play key roles in lignification and plant defense responses to hemipterans.

KW - Biotic stress

KW - Hemiptera

KW - Lignin

KW - Peroxidases

KW - ROS

KW - Switchgrass

UR - http://www.scopus.com/inward/record.url?scp=84899673872&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899673872&partnerID=8YFLogxK

U2 - 10.3389/fpls.2013.00202

DO - 10.3389/fpls.2013.00202

M3 - Review article

C2 - 23802005

AN - SCOPUS:84899673872

VL - 4

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

IS - JUN

M1 - 202

ER -