Towards the synthetic design of camelina oil enriched in tailored acetyl-triacylglycerols with medium-chain fatty acids

Sunil Bansal, Hae Jin Kim, Gun Nam Na, Megan E. Hamilton, Edgar B. Cahoon, Chaofu Lu, Timothy P. Durrett

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The ability to manipulate expression of key biosynthetic enzymes has allowed the development of genetically modified plants that synthesise unusual lipids that are useful for biofuel and industrial applications. By taking advantage of the unique activities of enzymes from different species, tailored lipids with a targeted structure can be conceived. In this study we demonstrate the successful implementation of such an approach by metabolically engineering the oilseed crop Camelina sativa to produce 3-acetyl-1,2-diacyl-sn-glycerols (acetyl-TAGs) with medium-chain fatty acids (MCFAs). Different transgenic camelina lines that had been genetically modified to produce MCFAs through the expression of MCFA-specific thioesterases and acyltransferases were retransformed with the Euonymus alatus gene for diacylglycerol acetyltransferase (EaDAcT) that synthesises acetyl-TAGs. Concomitant RNAi suppression of acyl-CoA:diacylglycerol acyltransferase increased the levels of acetyl-TAG, with up to 77 mole percent in the best lines. However, the total oil content was reduced. Analysis of the composition of the acetyl-TAG molecular species using electrospray ionisation mass spectrometry demonstrated the successful synthesis of acetyl-TAG containing MCFAs. Field growth of high-yielding plants generated enough oil for quantification of viscosity. As part of an ongoing design-test-learn cycle, these results, which include not only the synthesis of 'designer' lipids but also their functional analysis, will lead to the future production of such molecules tailored for specific applications.

Original languageEnglish (US)
Pages (from-to)4395-4402
Number of pages8
JournalJournal of experimental botany
Volume69
Issue number18
DOIs
StatePublished - Jan 1 2018

Fingerprint

Camelina
medium chain fatty acids
Oils
Triglycerides
Fatty Acids
triacylglycerols
oils
Lipids
Euonymus
Euonymus alatus
lipids
Diacylglycerol O-Acyltransferase
Camelina sativa
diacylglycerol acyltransferase
Acyltransferases
Acyl Coenzyme A
acyltransferases
acetyltransferases
Acetyltransferases
synthesis

Keywords

  • Acetyl-TAG
  • Biofuels
  • Bioproducts
  • Camelina sativa
  • Medium chain fatty acid
  • Synthetic biology
  • Vegetable oil

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Towards the synthetic design of camelina oil enriched in tailored acetyl-triacylglycerols with medium-chain fatty acids. / Bansal, Sunil; Kim, Hae Jin; Na, Gun Nam; Hamilton, Megan E.; Cahoon, Edgar B.; Lu, Chaofu; Durrett, Timothy P.

In: Journal of experimental botany, Vol. 69, No. 18, 01.01.2018, p. 4395-4402.

Research output: Contribution to journalArticle

Bansal, Sunil ; Kim, Hae Jin ; Na, Gun Nam ; Hamilton, Megan E. ; Cahoon, Edgar B. ; Lu, Chaofu ; Durrett, Timothy P. / Towards the synthetic design of camelina oil enriched in tailored acetyl-triacylglycerols with medium-chain fatty acids. In: Journal of experimental botany. 2018 ; Vol. 69, No. 18. pp. 4395-4402.
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