TNT biotransformation and detoxification by a Pseudomonas aeruginosa strain

Byung Taek Oh, Patrick J. Shea, Rhae A. Drijber, Galina K. Vasilyeva, Gautam Sarath

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Successful microbial-mediated remediation requires transformation pathways that maximize metabolism and minimize the accumulation of toxic products. Pseudomonas aeruginosa strain MX, isolated from munitions-contaminated soil, degraded 100 mg TNT L-1 in culture medium within 10 h under aerobic conditions. The major TNT products were 2-amino-4,6-dinitrotoluene (2ADNT, primarily in the supernatant) and 2,2′-azoxytoluene (2,2′AZT, primarily in the cell fraction), which accumulated as major products via the intermediate 2-hydroxylamino-4,6-dinitrotoluene (2HADNT). The 2HADNT and 2,2′AZT were relatively less toxic to the strain than TNT and 2ADNT. Aminodinitrotoluene (ADNT) production increased when yeast extract was added to the medium. While TNT transformation rate was not affected by pH, more HADNTs accumulated at pH 5.0 than at pH 8.0 and AZTs did not accumulate at the lower pH. The appearance of 2,6-diamino-4-nitrotoluene (2,6DANT) and 2,4-diamino-6-nitrotoluene (2,4DANT); dinitrotoluene (DNT) and nitrotoluene (NT); and 3,5-dinitroaniline (3,5DNA) indicated various routes of TNT metabolism and detoxification by P. aeruginosa strain MX.

Original languageEnglish (US)
Pages (from-to)309-319
Number of pages11
JournalBiodegradation
Volume14
Issue number5
DOIs
StatePublished - Oct 13 2003

Fingerprint

Trinitrotoluene
Detoxification
biotransformation
Biotransformation
detoxification
Pseudomonas aeruginosa
metabolism
Metabolism
oxic conditions
Poisons
yeast
remediation
Remediation
Yeast
Soils
Culture Media
Soil
Yeasts
product
rate

Keywords

  • Biotransformation
  • Detoxification
  • PH
  • Pseudomonas aeruginosa
  • TNT

ASJC Scopus subject areas

  • Environmental Engineering
  • Microbiology
  • Bioengineering
  • Environmental Chemistry
  • Pollution

Cite this

TNT biotransformation and detoxification by a Pseudomonas aeruginosa strain. / Oh, Byung Taek; Shea, Patrick J.; Drijber, Rhae A.; Vasilyeva, Galina K.; Sarath, Gautam.

In: Biodegradation, Vol. 14, No. 5, 13.10.2003, p. 309-319.

Research output: Contribution to journalArticle

Oh, Byung Taek ; Shea, Patrick J. ; Drijber, Rhae A. ; Vasilyeva, Galina K. ; Sarath, Gautam. / TNT biotransformation and detoxification by a Pseudomonas aeruginosa strain. In: Biodegradation. 2003 ; Vol. 14, No. 5. pp. 309-319.
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