TLR3 is an endogenous sensor of tissue necrosis during acute inflammatory events

Karen A. Cavassani, Makoto Ishii, Haitao Wen, Matthew A. Schaller, Pamela M. Lincoln, Nicholas W. Lukacs, Cory M. Hogaboam, Steven L. Kunkel

Research output: Contribution to journalArticle

311 Citations (Scopus)

Abstract

Ligands from dying cells are a source of Toll-like receptor (TLR) activating agents. Although TLR3 is known to respond to RNA from necrotic cells, the relative importance of this response in vivo during acute inflammatory processes has not been fully explored. We observed the involvement of TLR3 activation during experimental polymicrobial septic peritonitis and ischemic gut injury in the absence of an exogenous viral stimulus. In TLR3-deficient mice, increased chemokine/cytokine levels and neutrophil recruitment characterized the initial inflammatory responses in both injury models. However, the levels of inflammatory chemo- kines and tumor necrosis factor α; quickly returned to baseline in tlr3 -/- mice, and these mice were protected from the lethal effects of sustained inflammation. Macrophages from tlr3 -/- mice responded normally to other TLR ligands but did not respond to RNA from necrotic neutrophils. Importantly, an immunoneutralizing antibody directed against TLR3 attenuated the generation of inflammatory chemokines evoked by byproducts from necrotic neutrophils cultured with wild-type macrophages. In vivo, anti-TLR3 antibody attenuated the tissue injury associated with gut ischemia and significantly decreased sepsis-induced mortality. Collectively, these data show that TLR3 is a regulator of the amplification of immune response and serves an endogenous sensor of necrosis, independent of viral activation.

Original languageEnglish (US)
Pages (from-to)2609-2621
Number of pages13
JournalJournal of Experimental Medicine
Volume205
Issue number11
DOIs
StatePublished - Oct 27 2008

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Necrosis
Chemokines
Toll-Like Receptors
Wounds and Injuries
Neutrophils
Macrophages
RNA
Ligands
Virus Activation
Neutrophil Infiltration
Peritonitis
Anti-Idiotypic Antibodies
Sepsis
Ischemia
Tumor Necrosis Factor-alpha
Cytokines
Inflammation
Mortality
Antibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Cavassani, K. A., Ishii, M., Wen, H., Schaller, M. A., Lincoln, P. M., Lukacs, N. W., ... Kunkel, S. L. (2008). TLR3 is an endogenous sensor of tissue necrosis during acute inflammatory events. Journal of Experimental Medicine, 205(11), 2609-2621. https://doi.org/10.1084/jem.20081370

TLR3 is an endogenous sensor of tissue necrosis during acute inflammatory events. / Cavassani, Karen A.; Ishii, Makoto; Wen, Haitao; Schaller, Matthew A.; Lincoln, Pamela M.; Lukacs, Nicholas W.; Hogaboam, Cory M.; Kunkel, Steven L.

In: Journal of Experimental Medicine, Vol. 205, No. 11, 27.10.2008, p. 2609-2621.

Research output: Contribution to journalArticle

Cavassani, KA, Ishii, M, Wen, H, Schaller, MA, Lincoln, PM, Lukacs, NW, Hogaboam, CM & Kunkel, SL 2008, 'TLR3 is an endogenous sensor of tissue necrosis during acute inflammatory events', Journal of Experimental Medicine, vol. 205, no. 11, pp. 2609-2621. https://doi.org/10.1084/jem.20081370
Cavassani KA, Ishii M, Wen H, Schaller MA, Lincoln PM, Lukacs NW et al. TLR3 is an endogenous sensor of tissue necrosis during acute inflammatory events. Journal of Experimental Medicine. 2008 Oct 27;205(11):2609-2621. https://doi.org/10.1084/jem.20081370
Cavassani, Karen A. ; Ishii, Makoto ; Wen, Haitao ; Schaller, Matthew A. ; Lincoln, Pamela M. ; Lukacs, Nicholas W. ; Hogaboam, Cory M. ; Kunkel, Steven L. / TLR3 is an endogenous sensor of tissue necrosis during acute inflammatory events. In: Journal of Experimental Medicine. 2008 ; Vol. 205, No. 11. pp. 2609-2621.
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