Tissue factor

Identification and characterization of cell types in human placentae

W. P. Faulk, C. A. Labarrere, Steven D Carson

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

This is an immunohistologic study of tissue factor (TF) in snap frozen, unfixed, human normal-term placentae. Antibodies to TF were a monoclonal to human brain TF purified on a factor VII-agarose affinity column, and a polyclonal to a synthetic polypeptide representing the carboxyl-terminal nine amino acids of human TF. The results detail the localization and distribution of TF and characterize the cells in which it is found. TF was not observed in trophoblast, trophoblastic basement membranes, or noncellular components of connective tissue. TF was identified in some but not all macrophages, most fibroblast-like cells, and occasionally in perivascular cells and endothelium. The most consistent and intense reactions were obtained with vimentin-positive fibroblast-like cells in loose connective tissue. TF usually was not identified in fetal stem vessel endothelial cells, but TF reactivity was found in some of these cells in chorionic villi with histologic evidence of chronic inflammation. Such areas are uncommonly found in normal-term placentae. The vast majority of TF-reactive cells did not react with antibody to factor VII and were not in contact with blood. The biologic purpose of producing relatively great amounts of TF in areas remote from circulating factor VII is not known.

Original languageEnglish (US)
Pages (from-to)86-96
Number of pages11
JournalBlood
Volume76
Issue number1
StatePublished - Jan 1 1990

Fingerprint

Thromboplastin
Placenta
Factor VII
Fibroblasts
Connective Tissue
Endothelium
Tissue
Chorionic Villi
Antibodies
Macrophages
Endothelial cells
Trophoblasts
Vimentin
Basement Membrane
Sepharose
Brain
Blood
Endothelial Cells
Inflammation
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Tissue factor : Identification and characterization of cell types in human placentae. / Faulk, W. P.; Labarrere, C. A.; Carson, Steven D.

In: Blood, Vol. 76, No. 1, 01.01.1990, p. 86-96.

Research output: Contribution to journalArticle

Faulk, W. P. ; Labarrere, C. A. ; Carson, Steven D. / Tissue factor : Identification and characterization of cell types in human placentae. In: Blood. 1990 ; Vol. 76, No. 1. pp. 86-96.
@article{b1b62f38ebd5440f91fc0f1268a22240,
title = "Tissue factor: Identification and characterization of cell types in human placentae",
abstract = "This is an immunohistologic study of tissue factor (TF) in snap frozen, unfixed, human normal-term placentae. Antibodies to TF were a monoclonal to human brain TF purified on a factor VII-agarose affinity column, and a polyclonal to a synthetic polypeptide representing the carboxyl-terminal nine amino acids of human TF. The results detail the localization and distribution of TF and characterize the cells in which it is found. TF was not observed in trophoblast, trophoblastic basement membranes, or noncellular components of connective tissue. TF was identified in some but not all macrophages, most fibroblast-like cells, and occasionally in perivascular cells and endothelium. The most consistent and intense reactions were obtained with vimentin-positive fibroblast-like cells in loose connective tissue. TF usually was not identified in fetal stem vessel endothelial cells, but TF reactivity was found in some of these cells in chorionic villi with histologic evidence of chronic inflammation. Such areas are uncommonly found in normal-term placentae. The vast majority of TF-reactive cells did not react with antibody to factor VII and were not in contact with blood. The biologic purpose of producing relatively great amounts of TF in areas remote from circulating factor VII is not known.",
author = "Faulk, {W. P.} and Labarrere, {C. A.} and Carson, {Steven D}",
year = "1990",
month = "1",
day = "1",
language = "English (US)",
volume = "76",
pages = "86--96",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "1",

}

TY - JOUR

T1 - Tissue factor

T2 - Identification and characterization of cell types in human placentae

AU - Faulk, W. P.

AU - Labarrere, C. A.

AU - Carson, Steven D

PY - 1990/1/1

Y1 - 1990/1/1

N2 - This is an immunohistologic study of tissue factor (TF) in snap frozen, unfixed, human normal-term placentae. Antibodies to TF were a monoclonal to human brain TF purified on a factor VII-agarose affinity column, and a polyclonal to a synthetic polypeptide representing the carboxyl-terminal nine amino acids of human TF. The results detail the localization and distribution of TF and characterize the cells in which it is found. TF was not observed in trophoblast, trophoblastic basement membranes, or noncellular components of connective tissue. TF was identified in some but not all macrophages, most fibroblast-like cells, and occasionally in perivascular cells and endothelium. The most consistent and intense reactions were obtained with vimentin-positive fibroblast-like cells in loose connective tissue. TF usually was not identified in fetal stem vessel endothelial cells, but TF reactivity was found in some of these cells in chorionic villi with histologic evidence of chronic inflammation. Such areas are uncommonly found in normal-term placentae. The vast majority of TF-reactive cells did not react with antibody to factor VII and were not in contact with blood. The biologic purpose of producing relatively great amounts of TF in areas remote from circulating factor VII is not known.

AB - This is an immunohistologic study of tissue factor (TF) in snap frozen, unfixed, human normal-term placentae. Antibodies to TF were a monoclonal to human brain TF purified on a factor VII-agarose affinity column, and a polyclonal to a synthetic polypeptide representing the carboxyl-terminal nine amino acids of human TF. The results detail the localization and distribution of TF and characterize the cells in which it is found. TF was not observed in trophoblast, trophoblastic basement membranes, or noncellular components of connective tissue. TF was identified in some but not all macrophages, most fibroblast-like cells, and occasionally in perivascular cells and endothelium. The most consistent and intense reactions were obtained with vimentin-positive fibroblast-like cells in loose connective tissue. TF usually was not identified in fetal stem vessel endothelial cells, but TF reactivity was found in some of these cells in chorionic villi with histologic evidence of chronic inflammation. Such areas are uncommonly found in normal-term placentae. The vast majority of TF-reactive cells did not react with antibody to factor VII and were not in contact with blood. The biologic purpose of producing relatively great amounts of TF in areas remote from circulating factor VII is not known.

UR - http://www.scopus.com/inward/record.url?scp=0025334576&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025334576&partnerID=8YFLogxK

M3 - Article

VL - 76

SP - 86

EP - 96

JO - Blood

JF - Blood

SN - 0006-4971

IS - 1

ER -