Tissue factor encryption/de-encryption is not altered in the absence of the cytoplasmic domain

S. D. Carson, M. E. Bromberg

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Since the cytoplasmic domain of tissue factor (TF) appears to have a role in TF function beyond coagulation, experiments were conducted to determine whether the cytoplasmic domain also has a role in regulating procoagulant activity of TF present in the cell membrane. TF encryption was quantitated in human YU-SIT1, U87-MG, and mouse 3T3 cells which were transfected for expression of human tissue factor or a construct lacking the cytoplasmic domain (TF(CD)). Comparison of intact cells (encrypted) with fully disrupted cells (de-encrypted) showed that TF and TF(CD) were equally encrypted with respect to function in fX activation. Moreover, cells expressing TF and TF(CD) were indistinguishable in their procoagulant responses to A23187-calcium and varied concentrations of nonionic detergents. TF in membrane vesicles spontaneously shed by U87-MG cells was largely, but incompletely, de-encrypted, and the degree of de-encryption was independent of the cytoplasmic domain. We conclude that the predominant mechanism(s) for encrypting TF procoagulant activity is independent of the cytoplasmic domain.

Original languageEnglish (US)
Pages (from-to)657-663
Number of pages7
JournalThrombosis and Haemostasis
Volume84
Issue number4
StatePublished - Jan 1 2000

Fingerprint

Thromboplastin
3T3 Cells
Calcimycin
Detergents
Cell Membrane
Calcium

Keywords

  • Cytoplasmic domain
  • Encryption
  • Tissue factor

ASJC Scopus subject areas

  • Hematology

Cite this

Tissue factor encryption/de-encryption is not altered in the absence of the cytoplasmic domain. / Carson, S. D.; Bromberg, M. E.

In: Thrombosis and Haemostasis, Vol. 84, No. 4, 01.01.2000, p. 657-663.

Research output: Contribution to journalArticle

@article{e4b6e81a33a94c0383313c172b4399ec,
title = "Tissue factor encryption/de-encryption is not altered in the absence of the cytoplasmic domain",
abstract = "Since the cytoplasmic domain of tissue factor (TF) appears to have a role in TF function beyond coagulation, experiments were conducted to determine whether the cytoplasmic domain also has a role in regulating procoagulant activity of TF present in the cell membrane. TF encryption was quantitated in human YU-SIT1, U87-MG, and mouse 3T3 cells which were transfected for expression of human tissue factor or a construct lacking the cytoplasmic domain (TF(CD)). Comparison of intact cells (encrypted) with fully disrupted cells (de-encrypted) showed that TF and TF(CD) were equally encrypted with respect to function in fX activation. Moreover, cells expressing TF and TF(CD) were indistinguishable in their procoagulant responses to A23187-calcium and varied concentrations of nonionic detergents. TF in membrane vesicles spontaneously shed by U87-MG cells was largely, but incompletely, de-encrypted, and the degree of de-encryption was independent of the cytoplasmic domain. We conclude that the predominant mechanism(s) for encrypting TF procoagulant activity is independent of the cytoplasmic domain.",
keywords = "Cytoplasmic domain, Encryption, Tissue factor",
author = "Carson, {S. D.} and Bromberg, {M. E.}",
year = "2000",
month = "1",
day = "1",
language = "English (US)",
volume = "84",
pages = "657--663",
journal = "Thrombosis and Haemostasis",
issn = "0340-6245",
publisher = "Schattauer GmbH",
number = "4",

}

TY - JOUR

T1 - Tissue factor encryption/de-encryption is not altered in the absence of the cytoplasmic domain

AU - Carson, S. D.

AU - Bromberg, M. E.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Since the cytoplasmic domain of tissue factor (TF) appears to have a role in TF function beyond coagulation, experiments were conducted to determine whether the cytoplasmic domain also has a role in regulating procoagulant activity of TF present in the cell membrane. TF encryption was quantitated in human YU-SIT1, U87-MG, and mouse 3T3 cells which were transfected for expression of human tissue factor or a construct lacking the cytoplasmic domain (TF(CD)). Comparison of intact cells (encrypted) with fully disrupted cells (de-encrypted) showed that TF and TF(CD) were equally encrypted with respect to function in fX activation. Moreover, cells expressing TF and TF(CD) were indistinguishable in their procoagulant responses to A23187-calcium and varied concentrations of nonionic detergents. TF in membrane vesicles spontaneously shed by U87-MG cells was largely, but incompletely, de-encrypted, and the degree of de-encryption was independent of the cytoplasmic domain. We conclude that the predominant mechanism(s) for encrypting TF procoagulant activity is independent of the cytoplasmic domain.

AB - Since the cytoplasmic domain of tissue factor (TF) appears to have a role in TF function beyond coagulation, experiments were conducted to determine whether the cytoplasmic domain also has a role in regulating procoagulant activity of TF present in the cell membrane. TF encryption was quantitated in human YU-SIT1, U87-MG, and mouse 3T3 cells which were transfected for expression of human tissue factor or a construct lacking the cytoplasmic domain (TF(CD)). Comparison of intact cells (encrypted) with fully disrupted cells (de-encrypted) showed that TF and TF(CD) were equally encrypted with respect to function in fX activation. Moreover, cells expressing TF and TF(CD) were indistinguishable in their procoagulant responses to A23187-calcium and varied concentrations of nonionic detergents. TF in membrane vesicles spontaneously shed by U87-MG cells was largely, but incompletely, de-encrypted, and the degree of de-encryption was independent of the cytoplasmic domain. We conclude that the predominant mechanism(s) for encrypting TF procoagulant activity is independent of the cytoplasmic domain.

KW - Cytoplasmic domain

KW - Encryption

KW - Tissue factor

UR - http://www.scopus.com/inward/record.url?scp=0033790217&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033790217&partnerID=8YFLogxK

M3 - Article

C2 - 11057866

AN - SCOPUS:0033790217

VL - 84

SP - 657

EP - 663

JO - Thrombosis and Haemostasis

JF - Thrombosis and Haemostasis

SN - 0340-6245

IS - 4

ER -