Tissue factor antigen and activity are not expressed on the surface of intact cells isolated from an acute promyelocytic leukemia patient

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Tissue factor activity and antigen were measured in promyelocytes freshly isolated from a patient with acute promyelocytic leukemia (APL), FAB M3. Determination of functional activity revealed that physically disrupted cells expressed considerable tissue factor of which less than two percent was available prior to physical disruption of the cells. No tissue factor antigen was detectable on the cell surface by fluorescence flow cytometry. In contrast, endotoxin-stimulated peripheral blood monocytes and monoblastic cells isolated from a patient with monoblastic leukemia had notable populations of tissue factor-positive cells by flow cytometry, and expressed higher proportions of total tissue factor activity without disruption. While some cell types may express both tissue factor antigen and activity when intact, others, which can be extremely rich in tissue factor, may express neither antigen nor activity without a triggering event such as cell damage.

Original languageEnglish (US)
Pages (from-to)159-170
Number of pages12
JournalThrombosis Research
Volume59
Issue number1
DOIs
StatePublished - Jul 1 1990

Fingerprint

Acute Promyelocytic Leukemia
Thromboplastin
Antigens
Flow Cytometry
Granulocyte Precursor Cells
Endotoxins
Monocytes
Leukemia
Fluorescence
Population

Keywords

  • flow cytometry
  • leukemia
  • monocytes
  • tissue factor

ASJC Scopus subject areas

  • Hematology

Cite this

@article{c33b387d184d49ea90bb071ea83e86ae,
title = "Tissue factor antigen and activity are not expressed on the surface of intact cells isolated from an acute promyelocytic leukemia patient",
abstract = "Tissue factor activity and antigen were measured in promyelocytes freshly isolated from a patient with acute promyelocytic leukemia (APL), FAB M3. Determination of functional activity revealed that physically disrupted cells expressed considerable tissue factor of which less than two percent was available prior to physical disruption of the cells. No tissue factor antigen was detectable on the cell surface by fluorescence flow cytometry. In contrast, endotoxin-stimulated peripheral blood monocytes and monoblastic cells isolated from a patient with monoblastic leukemia had notable populations of tissue factor-positive cells by flow cytometry, and expressed higher proportions of total tissue factor activity without disruption. While some cell types may express both tissue factor antigen and activity when intact, others, which can be extremely rich in tissue factor, may express neither antigen nor activity without a triggering event such as cell damage.",
keywords = "flow cytometry, leukemia, monocytes, tissue factor",
author = "Carson, {Steven D} and Pirruccello, {Samuel Jay} and Haire, {William D.}",
year = "1990",
month = "7",
day = "1",
doi = "10.1016/0049-3848(90)90281-G",
language = "English (US)",
volume = "59",
pages = "159--170",
journal = "Thrombosis Research",
issn = "0049-3848",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Tissue factor antigen and activity are not expressed on the surface of intact cells isolated from an acute promyelocytic leukemia patient

AU - Carson, Steven D

AU - Pirruccello, Samuel Jay

AU - Haire, William D.

PY - 1990/7/1

Y1 - 1990/7/1

N2 - Tissue factor activity and antigen were measured in promyelocytes freshly isolated from a patient with acute promyelocytic leukemia (APL), FAB M3. Determination of functional activity revealed that physically disrupted cells expressed considerable tissue factor of which less than two percent was available prior to physical disruption of the cells. No tissue factor antigen was detectable on the cell surface by fluorescence flow cytometry. In contrast, endotoxin-stimulated peripheral blood monocytes and monoblastic cells isolated from a patient with monoblastic leukemia had notable populations of tissue factor-positive cells by flow cytometry, and expressed higher proportions of total tissue factor activity without disruption. While some cell types may express both tissue factor antigen and activity when intact, others, which can be extremely rich in tissue factor, may express neither antigen nor activity without a triggering event such as cell damage.

AB - Tissue factor activity and antigen were measured in promyelocytes freshly isolated from a patient with acute promyelocytic leukemia (APL), FAB M3. Determination of functional activity revealed that physically disrupted cells expressed considerable tissue factor of which less than two percent was available prior to physical disruption of the cells. No tissue factor antigen was detectable on the cell surface by fluorescence flow cytometry. In contrast, endotoxin-stimulated peripheral blood monocytes and monoblastic cells isolated from a patient with monoblastic leukemia had notable populations of tissue factor-positive cells by flow cytometry, and expressed higher proportions of total tissue factor activity without disruption. While some cell types may express both tissue factor antigen and activity when intact, others, which can be extremely rich in tissue factor, may express neither antigen nor activity without a triggering event such as cell damage.

KW - flow cytometry

KW - leukemia

KW - monocytes

KW - tissue factor

UR - http://www.scopus.com/inward/record.url?scp=0025117992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025117992&partnerID=8YFLogxK

U2 - 10.1016/0049-3848(90)90281-G

DO - 10.1016/0049-3848(90)90281-G

M3 - Article

VL - 59

SP - 159

EP - 170

JO - Thrombosis Research

JF - Thrombosis Research

SN - 0049-3848

IS - 1

ER -