Time dependent effects of restraint stress on food intake in rats

I. I. Rybkin, Y. Zhou, G. N. Smagin, D. H. Ryan, R. B.S. Harris

Research output: Contribution to journalArticle

Abstract

We investigated whether 3 hours restraint stress (RS) applied at different times of the light cycle produced differential effects on food intake and body weight. Male Sprague Dawley rats were divided into 4 weight matched groups: Exp. 1-control and RS with stress from 4:00 to 7:00 pm and Exp. 2-control and RS with stress from 8:00 to 11:00 am (lights off 7:00 pm to 7:00 am). Food and water intake, and body weight were measured during three periods: 7 days baseline, day of RS, and 11 days of recovery. On the day of stress food and water intake were measured at intervals. RS significantly inhibited 24 hour food, but not water, intake. In Exp. 1 food intake was suppressed during the first 2 hours of the dark cycle. In Exp. 2 intake was suppressed for 4 hours immediately after RS and during the first 2 hours of the dark cycle. There was no effect of RS on intake during recovery. There was no significant effect of RS on body weight in Exp. 1. In Exp.2 RS rats weighed less than controls throughout recovery. After recovery treatment groups were switched (i.e. control were exposed to RS). Measurements made within the first hour of the dark cycle revealed no significant differences in hypothalamic NPY mRNA expression. NPY protein in the paraventricular nucleus of hypothalamus was significantly higher in RS rats from Exp. 2, compared with their controls or RS rats from Exp. 1. There were no obvious Rs-induced changes in hypothalamic monoamine concentration. RS has greater effect on metabolism and energy balance when it is applied in the morning.

Original languageEnglish (US)
Pages (from-to)A173
JournalFASEB Journal
Volume11
Issue number3
StatePublished - Dec 1 1997

Fingerprint

Rats
food intake
Eating
Drinking
rats
Body Weight
body weight
paraventricular hypothalamic nucleus
monoamines
Paraventricular Hypothalamic Nucleus
water
Photoperiod
energy balance
Energy Metabolism
Hypothalamus
Sprague Dawley Rats
Research Design
photoperiod
Light
Weights and Measures

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Rybkin, I. I., Zhou, Y., Smagin, G. N., Ryan, D. H., & Harris, R. B. S. (1997). Time dependent effects of restraint stress on food intake in rats. FASEB Journal, 11(3), A173.

Time dependent effects of restraint stress on food intake in rats. / Rybkin, I. I.; Zhou, Y.; Smagin, G. N.; Ryan, D. H.; Harris, R. B.S.

In: FASEB Journal, Vol. 11, No. 3, 01.12.1997, p. A173.

Research output: Contribution to journalArticle

Rybkin, II, Zhou, Y, Smagin, GN, Ryan, DH & Harris, RBS 1997, 'Time dependent effects of restraint stress on food intake in rats', FASEB Journal, vol. 11, no. 3, pp. A173.
Rybkin II, Zhou Y, Smagin GN, Ryan DH, Harris RBS. Time dependent effects of restraint stress on food intake in rats. FASEB Journal. 1997 Dec 1;11(3):A173.
Rybkin, I. I. ; Zhou, Y. ; Smagin, G. N. ; Ryan, D. H. ; Harris, R. B.S. / Time dependent effects of restraint stress on food intake in rats. In: FASEB Journal. 1997 ; Vol. 11, No. 3. pp. A173.
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